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RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois

Overview of attention for article published in Veterinary Research, June 2018
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3 tweeters

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Title
RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois
Published in
Veterinary Research, June 2018
DOI 10.1186/s13567-018-0546-6
Pubmed ID
Authors

Naíla C. do Nascimento, Ana M. S. Guimaraes, Andrea P. dos Santos, Yuefeng Chu, Lucas M. Marques, Joanne B. Messick

Abstract

Pigs are popular animal models in biomedical research. RNA-Seq is becoming the predominant tool to investigate transcriptional changes of the pig's response to infection. The high sensitivity of this tool requires a strict control of the study design beginning with the selection of healthy animals to provide accurate interpretation of research data. Pigs chronically infected with Mycoplasma suis often show no obvious clinical signs, however the infection may affect the validity of animal research. The goal of this study was to investigate whether or not this silent infection is also silent at the host transcriptional level. Therefore, immunocompetent pigs were experimentally infected with M. suis and transcriptional profiles of whole blood, generated by RNA-Seq, were analyzed and compared to non-infected animals. RNA-Seq showed 55 differentially expressed (DE) genes in the M. suis infected pigs. Down-regulation of genes related to innate immunity (tlr8, chemokines, chemokines receptors) and genes containing IFN gamma-activated sequence (gbp1, gbp2, il15, cxcl10, casp1, cd274) suggests a general suppression of the immune response in the infected animals. Sixteen (29.09%) of the DE genes were involved in two protein interaction networks: one involving chemokines, chemokine receptors and interleukin-15 and another involving the complement cascade. Genes related to vascular permeability, blood coagulation, and endothelium integrity were also DE in infected pigs. These findings suggest that M. suis subclinical infection causes significant alterations in blood mRNA levels, which could impact data interpretation of research using pigs. Screening of pigs for M. suis infection before initiating animal studies is strongly recommended.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 24%
Student > Bachelor 4 19%
Researcher 4 19%
Student > Doctoral Student 2 10%
Professor 1 5%
Other 2 10%
Unknown 3 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 24%
Veterinary Science and Veterinary Medicine 4 19%
Agricultural and Biological Sciences 4 19%
Psychology 2 10%
Business, Management and Accounting 1 5%
Other 2 10%
Unknown 3 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2018.
All research outputs
#10,420,424
of 13,640,418 outputs
Outputs from Veterinary Research
#599
of 845 outputs
Outputs of similar age
#185,930
of 268,440 outputs
Outputs of similar age from Veterinary Research
#1
of 1 outputs
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So far Altmetric has tracked 845 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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