Title |
Changes in microbiota during experimental human Rhinovirus infection
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Published in |
BMC Infectious Diseases, August 2015
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DOI | 10.1186/s12879-015-1081-y |
Pubmed ID | |
Authors |
J. J. Hofstra, S. Matamoros, M. A. van de Pol, B. de Wever, M. W. Tanck, H. Wendt-Knol, M. Deijs, L. van der Hoek, K. C. Wolthers, R. Molenkamp, C. E. Visser, P. J. Sterk, R. Lutter, M. D. de Jong |
Abstract |
Human Rhinovirus (HRV) is responsible for the majority of common colds and is frequently accompanied by secondary bacterial infections through poorly understood mechanisms. We investigated the effects of experimental human HRV serotype 16 infection on the upper respiratory tract microbiota. Six healthy volunteers were infected with HRV16. We performed 16S ribosomal RNA-targeted pyrosequencing on throat swabs taken prior, during and after infection. We compared overall community diversity, phylogenetic structure of the ecosystem and relative abundances of the different bacteria between time points. During acute infection strong trends towards increases in the relative abundances of Haemophilus parainfluenzae and Neisseria subflava were observed, as well as a weaker trend towards increases of Staphylococcus aureus. No major differences were observed between day-1 and day 60, whereas differences between subjects were very high. HRV16 infection is associated with the increase of three genera known to be associated with secondary infections following HRV infections. The observed changes of upper respiratory tract microbiota could help explain why HRV infection predisposes to bacterial otitis media, sinusitis and pneumonia. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 3 | 38% |
United Kingdom | 1 | 13% |
Unknown | 4 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 6 | 75% |
Practitioners (doctors, other healthcare professionals) | 2 | 25% |
Mendeley readers
Geographical breakdown
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United States | 1 | 1% |
Unknown | 85 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 24 | 28% |
Student > Master | 9 | 10% |
Student > Bachelor | 9 | 10% |
Student > Ph. D. Student | 7 | 8% |
Professor > Associate Professor | 5 | 6% |
Other | 14 | 16% |
Unknown | 18 | 21% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 17 | 20% |
Medicine and Dentistry | 17 | 20% |
Nursing and Health Professions | 4 | 5% |
Biochemistry, Genetics and Molecular Biology | 3 | 3% |
Other | 8 | 9% |
Unknown | 20 | 23% |