Title |
Suppression of miR-181a attenuates H2O2-induced death of mesenchymal stem cells by maintaining hexokinase II expression
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Published in |
Biological Research, August 2015
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DOI | 10.1186/s40659-015-0036-5 |
Pubmed ID | |
Authors |
Seahyoung Lee, Ina Yun, Onju Ham, Se-Yeon Lee, Chang Yeon Lee, Jun-Hee Park, Jiyun Lee, Hyang-Hee Seo, Eunhyun Choi, Ki-Chul Hwang |
Abstract |
Low survival rate of transplanted cells compromises the efficacy of cell therapy. Hexokinase II (HKII) is known to have anti-apoptotic activity through its interaction with mitochondria. The objective was to identify miRNAs targeting HKII and investigate whether miRNA-mediated modulation of HKII could improve the survival of mesenchymal stem cells (MSCs) exposed to H2O2. The expression of HKII in MSCs exposed to H2O2 was evaluated, and HKII-targeting miRNA was screened based on miRNA-target prediction databases. The effect of H2O2 on the expression of the selected HKII-targeting miRNA was examined and the effect of modulation of the selected HKII-targeting miRNA using anti-miRNA on H2O2-induced apoptosis of MSC was evaluated. H2O2 (600 μM) induced cell death of MSCs and decreased mitochondrial HKII expression. We have identified miR-181a as a HKII-targeting miRNA and H2O2 increased the expression of miR-181a in MSCs. Delivery of anti-miR-181a, which neutralizes endogenous miR-181a, significantly attenuated H2O2-induced decrease of HKII expression and disruption of mitochondrial membrane potential, improving the survival of MSCs exposed to H2O2. These findings suggest that H2O2-induced up-regulation of miR-181a contributes to the cell death of MSCs by down-regulating HKII. Neutralizing miR-181a can be an effective way to prime MSCs for transplantation into ischemic tissues. |
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Unknown | 2 | 100% |
Demographic breakdown
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 4% |
Unknown | 23 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 25% |
Researcher | 6 | 25% |
Student > Bachelor | 4 | 17% |
Other | 2 | 8% |
Lecturer > Senior Lecturer | 1 | 4% |
Other | 2 | 8% |
Unknown | 3 | 13% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 17% |
Medicine and Dentistry | 4 | 17% |
Agricultural and Biological Sciences | 3 | 13% |
Neuroscience | 2 | 8% |
Psychology | 2 | 8% |
Other | 3 | 13% |
Unknown | 6 | 25% |