Title |
RNAseq analysis of hippocampal microglia after kainic acid-induced seizures
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Published in |
Molecular Brain, June 2018
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DOI | 10.1186/s13041-018-0376-5 |
Pubmed ID | |
Authors |
Dale B. Bosco, Jiaying Zheng, Zhiyan Xu, Jiyun Peng, Ukpong B. Eyo, Ke Tang, Cheng Yan, Jun Huang, Lijie Feng, Gongxiong Wu, Jason R. Richardson, Hui Wang, Long-Jun Wu |
Abstract |
Microglia have been shown to be of critical importance to the progression of temporal lobe epilepsy. However, the broad transcriptional changes that these cells undergo following seizure induction is not well understood. As such, we utilized RNAseq analysis upon microglia isolated from the hippocampus to determine expression pattern alterations following kainic acid induced seizure. We determined that microglia undergo dramatic changes to their expression patterns, particularly with regard to mitochondrial activity and metabolism. We also observed that microglia initiate immunological activity, specifically increasing interferon beta responsiveness. Our results provide novel insights into microglia transcriptional regulation following acute seizures and suggest potential therapeutic targets specifically in microglia for the treatment of seizures and epilepsy. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 67 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 15 | 22% |
Researcher | 8 | 12% |
Student > Master | 6 | 9% |
Student > Doctoral Student | 5 | 7% |
Student > Bachelor | 4 | 6% |
Other | 12 | 18% |
Unknown | 17 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 22 | 33% |
Biochemistry, Genetics and Molecular Biology | 10 | 15% |
Immunology and Microbiology | 4 | 6% |
Agricultural and Biological Sciences | 3 | 4% |
Nursing and Health Professions | 2 | 3% |
Other | 6 | 9% |
Unknown | 20 | 30% |