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MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesis

Overview of attention for article published in BMC Immunology, June 2018
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Title
MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesis
Published in
BMC Immunology, June 2018
DOI 10.1186/s12865-018-0255-y
Pubmed ID
Authors

Sawang Petvises, Pravin Periasamy, Helen C. O’Neill

Abstract

Splenic stroma overlaid with hematopoietic progenitors supports in vitro hematopoiesis with production of dendritic-like cells. Co-cultures of murine lineage-depleted bone marrow over the 5G3 stromal line produce two populations of cells, characterised as CD11b+CD11c+MHC-II- dendritic-like 'L-DC', and CD11b+CD11c+MHC-II+ cells, resembling conventional dendritic cells (cDC). To date, the functional capacity of these two subsets has not been clearly distinguished. Here we show both the L-DC and cDC-like subsets can be activated and induce proliferation of OT-I CD8+ T cells, being strong inducers of IL-2 and IFN-γ production. Both subsets lack ability to induce proliferation of OT-II CD4+ T cells. The cDC-like population is shown here to resemble regulatory DC in that they induce FoxP3 expression and IL-10 production in OT-II CD4+ T cells, in line with their function as regulatory DC. L-DC did not activate or induce the proliferation of CD4+ T cells and did not induce FoxP3 expression in CD4+ T cells. L-DC can be distinguished from cDC-like cells through their superior endocytic capacity and expression of 4-1BBL, F4/80 and Sirp-α. A comparison of gene expression by the two subsets was consistent with L-DC having an activated or immunostimulatory DC phenotype, while cDC-like cells reflect myeloid dendritic cells with inflammatory and suppressive properties, also consistent with functional characteristics as regulatory DC. When a Transwell membrane was used to prevent hematopoietic cell contact with stroma, only cDC-like cells and not L-DC were produced, and cell production was dependent on M-CSF production by stroma. Co-cultures of hematopoietic progenitors over splenic stroma produce two distinct subsets of dendritic-like cells. These are here distinguished phenotypically and through gene expression differences. While both resemble DC, there are functionally distinct. L-DC activate CD8+ but not CD4+ T cells, while the cDC-like population induce regulatory T cells, so reflecting regulatory DC. The latter can be enriched through Transwell co-cultures with cell production dependent on M-CSF.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 43%
Researcher 3 43%
Student > Bachelor 1 14%
Readers by discipline Count As %
Immunology and Microbiology 2 29%
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 1 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 14%
Medicine and Dentistry 1 14%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 June 2018.
All research outputs
#9,229,757
of 15,075,497 outputs
Outputs from BMC Immunology
#238
of 480 outputs
Outputs of similar age
#154,743
of 276,026 outputs
Outputs of similar age from BMC Immunology
#1
of 1 outputs
Altmetric has tracked 15,075,497 research outputs across all sources so far. This one is in the 36th percentile – i.e., 36% of other outputs scored the same or lower than it.
So far Altmetric has tracked 480 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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