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Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii

Overview of attention for article published in Plant Methods, November 2011
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1 tweeter

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Title
Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
Published in
Plant Methods, November 2011
DOI 10.1186/1746-4811-7-35
Pubmed ID
Authors

Daniela Strenkert, Stefan Schmollinger, Michael Schroda

Abstract

We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Norway 1 1%
United Kingdom 1 1%
Mexico 1 1%
Spain 1 1%
United States 1 1%
Unknown 65 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 22 31%
Researcher 16 23%
Student > Master 8 11%
Student > Bachelor 6 9%
Student > Postgraduate 4 6%
Other 8 11%
Unknown 6 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 41 59%
Biochemistry, Genetics and Molecular Biology 18 26%
Business, Management and Accounting 1 1%
Environmental Science 1 1%
Social Sciences 1 1%
Other 1 1%
Unknown 7 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 November 2011.
All research outputs
#12,357,523
of 18,796,975 outputs
Outputs from Plant Methods
#672
of 894 outputs
Outputs of similar age
#83,443
of 126,444 outputs
Outputs of similar age from Plant Methods
#10
of 18 outputs
Altmetric has tracked 18,796,975 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 894 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 17th percentile – i.e., 17% of its peers scored the same or lower than it.
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We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.