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Identification of commercial Ganoderma (Lingzhi) species by ITS2 sequences

Overview of attention for article published in Chinese Medicine, August 2015
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  • Above-average Attention Score compared to outputs of the same age (54th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

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Title
Identification of commercial Ganoderma (Lingzhi) species by ITS2 sequences
Published in
Chinese Medicine, August 2015
DOI 10.1186/s13020-015-0056-7
Pubmed ID
Authors

Baosheng Liao, Xiaochen Chen, Jianping Han, Yang Dan, Lili Wang, Wenjing Jiao, Jingyuan Song, Shilin Chen

Abstract

DNA barcoding can be used to authenticate Ganoderma species for safe use. This study aims to identify commercial products containing Ganoderma using DNA barcoding. We used 63 internal transcribed spacer (ITS) 2 sequences of Ganoderma species from 33 newly-sequenced wild samples, crude drugs, mycelia, spores, and authentic extracts and spore oils collected from various locations and markets, as well as 30 sequences from GenBank. Sequences were assembled and aligned using CodonCode Aligner V3.71. Intra- and inter-specific distances were estimated by MEGA 6.0, and phylogeny reconstruction was based on the K2P model. SNP(s) and RNA secondary structure of ITS2 were analyzed and compared among closely related Ganoderma species. G. lucidum cultivated in China was different from those cultivated in Europe. "Chizhi" (G. lucidum) and "Zizhi" (G. sinense) were clustered into two clades that were separated from the other Ganoderma species. The fruiting bodies and commercial products of G. lucidum and G. sinense were successfully distinguished from those of other Ganoderma species by comparing the ITS2 sequences and RNA secondary structures. The DNA barcoding method is applicable to the authentication of commercial products containing Ganoderma species.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 48 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 19%
Researcher 8 17%
Student > Ph. D. Student 4 8%
Student > Doctoral Student 3 6%
Lecturer 2 4%
Other 5 10%
Unknown 17 35%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 23%
Medicine and Dentistry 5 10%
Biochemistry, Genetics and Molecular Biology 5 10%
Pharmacology, Toxicology and Pharmaceutical Science 3 6%
Environmental Science 1 2%
Other 5 10%
Unknown 18 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2015.
All research outputs
#14,473,281
of 25,373,627 outputs
Outputs from Chinese Medicine
#199
of 660 outputs
Outputs of similar age
#126,550
of 277,605 outputs
Outputs of similar age from Chinese Medicine
#1
of 10 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 660 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,605 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them