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Sheep embryonic stem-like cells engrafted into sheep femoral condyle osteochondral defects: 4-year follow-up

Overview of attention for article published in BMC Veterinary Research, June 2018
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3 tweeters

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Title
Sheep embryonic stem-like cells engrafted into sheep femoral condyle osteochondral defects: 4-year follow-up
Published in
BMC Veterinary Research, June 2018
DOI 10.1186/s12917-018-1532-y
Pubmed ID
Authors

Susanna Pilichi, Stefano Rocca, Maria Dattena, Roy Ransom Pool, Laura Mara, Daniela Sanna, Gerolamo Masala, Maria Lucia Manunta, Simone Dore, Andrea Manunta, Eraldo Sanna Passino

Abstract

Articular cartilage lacks a regenerative response. Embryonic stem cells (ESCs) are a source of pluripotent cells for cartilage regeneration. Their use, however, is associated with a risk of teratoma development, which depends on multiple factors including the number of engrafted cells and their degree of histocompatibility with recipients, the immunosuppression of the host and the site of transplantation. Colonies of sheep embryonic stem-like (ES-like) cells from in vitro-produced embryos, positive for stage-specific embryonic antigens (SSEAs), alkaline phosphatase (ALP), Oct 4, Nanog, Sox 2 and Stat 3 gene expression, and forming embryoid bodies, were pooled in groups of two-three, embedded in fibrin glue and engrafted into osteochondral defects in the left medial femoral condyles of 3 allogeneic ewes (ES). Empty defects (ED) and defects filled with cell-free glue (G) in the condyles of the controlateral stifle joint served as controls. After euthanasia at 4 years post-engraftment, the regenerated tissue was evaluated by macroscopic, histological and immunohistochemical (collagen type II) examinations and fluorescent in situ hybridization (FISH) assay to prove the ES-like cells origin of the regenerated tissue. No teratoma occurred in any of the ES samples. No statistically significant macroscopic or histological differences were observed among the 3 treatment groups. FISH was positive in all the 3 ES samples. This in vivo preclinical study allowed a long-term evaluation of the occurrence of teratoma in non-immunosuppressed allogeneic adult sheep engrafted with allogeneic ES-like cells, supporting the safe and reliable application of ES cells in the clinic.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 14%
Researcher 2 14%
Student > Doctoral Student 1 7%
Lecturer > Senior Lecturer 1 7%
Professor > Associate Professor 1 7%
Other 0 0%
Unknown 7 50%
Readers by discipline Count As %
Medicine and Dentistry 3 21%
Veterinary Science and Veterinary Medicine 1 7%
Biochemistry, Genetics and Molecular Biology 1 7%
Engineering 1 7%
Unknown 8 57%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2018.
All research outputs
#10,402,258
of 13,615,090 outputs
Outputs from BMC Veterinary Research
#1,095
of 1,999 outputs
Outputs of similar age
#185,375
of 267,603 outputs
Outputs of similar age from BMC Veterinary Research
#2
of 2 outputs
Altmetric has tracked 13,615,090 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,999 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,603 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one.