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Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations

Overview of attention for article published in BMC Biotechnology, August 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#31 of 888)
  • High Attention Score compared to outputs of the same age (90th percentile)

Mentioned by

news
1 news outlet
blogs
1 blog
twitter
2 tweeters
peer_reviews
1 peer review site

Citations

dimensions_citation
29 Dimensions

Readers on

mendeley
65 Mendeley
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Title
Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations
Published in
BMC Biotechnology, August 2015
DOI 10.1186/s12896-015-0180-6
Pubmed ID
Authors

Afshin Hassani-Mehraban, Sjoerd Creutzburg, Luc van Heereveld, Richard Kormelink

Abstract

Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit vaccines. To test the feasibility of Cowpea chlorotic mottle virus (CCMV) particles as a scaffold for epitope presentation and identify sites for epitope fusion or insertion that would not interfere with virus-like-particle formation, chimeric CCMV coat protein (CP) gene constructs were engineered, followed by expression in E. coli and assessment of VLP formation. Various constructs were made encoding a 6x-His-tag, or selected epitopes from Influenza A virus [IAV] (M2e, HA) or Foot and Mouth Disease Virus [FMDV] (VP1 and 2C). The epitopes were either inserted 1) in predicted exposed loop structures of the CCMV CP protein, 2) fused to the amino- (N) or carboxyl-terminal (C) ends, or 3) to a N-terminal 24 amino acid (aa) deletion mutant (N∆24-CP) of the CP protein. High levels of insoluble protein expression, relative to proteins from the entire cell lysate, were obtained for CCMV CP and all chimeric derivatives. A straightforward protocol was used that, without the use of purification columns, successfully enabled CCMV CP protein solubilization, reassembly and subsequent collection of CCMV CP VLPs. While insertions of His-tag or M2e (7-23 aa) into the predicted external loop structures did abolish VLP formation, high yields of VLPs were obtained with all fusions of His-tag or various epitopes (13- 27 aa) from IAV and FMDV at the N- or C-terminal ends of CCMV CP or N∆24-CP. VLPs derived from CCMV CP still encapsulated RNA, while those from CCMV CP-chimera containing a negatively charged N-terminal domain had lost this ability. The usefulness and rapid ease of exploitation of CCMV VLPs for the production of potential subunit vaccines was demonstrated with the synthesis of chimeric CCMV VLPs containing selected sequences from the GN and GC glycoproteins of the recently emerged Schmallenberg orthobunyavirus at both termini of the CP protein. CCMV VLPs can be successfully exploited as scaffold for epitope fusions up to 31 aa at the N- and C-terminus, and at a N-terminal 24 amino acid (aa) deletion mutant (N∆24-CP) of the CP protein.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
South Africa 1 2%
Unknown 63 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 26%
Student > Master 13 20%
Researcher 7 11%
Student > Bachelor 6 9%
Student > Doctoral Student 3 5%
Other 5 8%
Unknown 14 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 16 25%
Agricultural and Biological Sciences 12 18%
Immunology and Microbiology 5 8%
Chemistry 4 6%
Engineering 4 6%
Other 9 14%
Unknown 15 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 December 2020.
All research outputs
#1,559,804
of 19,546,162 outputs
Outputs from BMC Biotechnology
#31
of 888 outputs
Outputs of similar age
#24,435
of 249,423 outputs
Outputs of similar age from BMC Biotechnology
#1
of 1 outputs
Altmetric has tracked 19,546,162 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 888 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 249,423 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them