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Improved serological detection of rheumatoid arthritis: a highly antigenic mimotope of carbonic anhydrase III selected in a murine model by phage display

Overview of attention for article published in Arthritis Research & Therapy, June 2015
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56 Mendeley
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Title
Improved serological detection of rheumatoid arthritis: a highly antigenic mimotope of carbonic anhydrase III selected in a murine model by phage display
Published in
Arthritis Research & Therapy, June 2015
DOI 10.1186/s13075-015-0685-3
Pubmed ID
Authors

Galber Rodrigues Araujo, Emília Rezende Vaz, Patricia Tiemi Fujimura, João Eurico Fonseca, Lucélia Maria de Lima, Helena Canhão, Gabriela Venturini, Karina Helena Morais Cardozo, Valdemir Melechco Carvalho, Marcelo Henrique Napimoga, Luiz Ricardo Goulart, João Gonçalves, Carlos Ueira-Vieira

Abstract

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that affects around 1 % of the human population worldwide. RA diagnosis can be difficult by the fact that there is no definitive test for its detection. Therefore, the aim of this study was to identify biomarkers that could be used for RA diagnosis. Sera from collagen-induced arthritis (CIA) mouse model were used to select potential biomarkers for RA diagnosis by Phage Display technology. In silico and in vitro analyses were performed to characterize and validate the selected peptides. Samples were classified into three groups: RA, two other immune-mediated rheumatic diseases (systemic lupus erythematosus - SLE and ankylosing spondylitis - AS) and healthy controls (HC). ELISA assay was carried out to determine antibody levels, and diagnostic parameters were determined by constructing Receiver Operating Characteristic (ROC) curves. Mass spectrometry and western blot were performed to identify the putative autoantigen that was mimicked by a highly reactive mimotope. After three rounds of selection, fourteen clones were obtained and tested for immunoreactivity analysis against sera from RA and HC groups. The phage-fused peptide with the highest immunoreactivity (M12) was synthesized, and was able of efficiently discriminate RA patients from SLE, AS and HCs (p < 0.0001) by ELISA. The specificity and sensitivity of anti-M12 antibodies for RA diagnosis were 91 % and 84.3 %, respectively. The M12 peptide was identified as a peptide that mimics a predicted antigenic site of the carbonic anhydrase III (CAIII) protein, a ubiquitous biomarker that has been identified in patients with other diseases. M12 is the first peptide associated to CAIII protein that may be used as an antigen for antibodies detection to aid in RA diagnosis with high sensitivity and specificity.

Mendeley readers

The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Unknown 55 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 18%
Researcher 7 13%
Student > Bachelor 6 11%
Student > Ph. D. Student 6 11%
Librarian 4 7%
Other 13 23%
Unknown 10 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 20%
Chemistry 6 11%
Agricultural and Biological Sciences 5 9%
Medicine and Dentistry 5 9%
Immunology and Microbiology 4 7%
Other 13 23%
Unknown 12 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 June 2015.
All research outputs
#7,813,294
of 12,451,992 outputs
Outputs from Arthritis Research & Therapy
#1,414
of 1,983 outputs
Outputs of similar age
#126,900
of 239,587 outputs
Outputs of similar age from Arthritis Research & Therapy
#44
of 74 outputs
Altmetric has tracked 12,451,992 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,983 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 239,587 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 74 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.