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Development of a simple procedure for the treatment of femoral head osteonecrosis with intra-osseous injection of bone marrow mesenchymal stromal cells: study of their biodistribution in the early…

Overview of attention for article published in Stem Cell Research & Therapy, April 2015
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1 tweeter

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36 Dimensions

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Title
Development of a simple procedure for the treatment of femoral head osteonecrosis with intra-osseous injection of bone marrow mesenchymal stromal cells: study of their biodistribution in the early time points after injection
Published in
Stem Cell Research & Therapy, April 2015
DOI 10.1186/s13287-015-0036-y
Pubmed ID
Authors

Angélique Lebouvier, Alexandre Poignard, Madeleine Cavet, Jérôme Amiaud, Julie Leotot, Philippe Hernigou, Alain Rahmouni, Philippe Bierling, Pierre Layrolle, Hélène Rouard, Nathalie Chevallier

Abstract

Osteonecrosis of the femoral head (ONFH) is a degenerative disease progressing to a femoral head (FH) collapse. Injection of osteoprogenitor cells like bone marrow mesenchymal stromal cells (BMSCs) into the FH appears to be a good therapeutic treatment. However, safety and efficacy of BMSCs to treat bone defect are the main preclinical data required for clinical application. Efficacy and no risk of cells transformation after amplification of BMSCs have been extensively described. The main objectives of this study were to develop a simple and usable procedure for clinicians and control its feasibility by evaluating the biodistribution of BMSCs after injection into the FH in a large animal model. The impact of this approach was evaluated on one natural pig ONFH. BMSCs were directly injected in the pig FH, then the biodistribution of grafted cells were detected either by qPCR, cytometry or by combination of classical histology analysis and in situ hybridization (ISH). BMSCs' efficacy on bone regeneration was evaluated by MRI and histology. After 30 min and 24 h follow-up, grafted cells were detected at the injection site and no BMSCs were detected in filters organs or body fluids. Combination of classical histology analysis and ISH showed a good homogeneity of cells distribution in FH. Local delivery of BMSCs onto a bone scaffold associated to bone formation in vivo confirmed the preferential tropism of BMSCs to the bone tissue as well as their efficacy to form bone. Treatment of a natural pig ONFH by autologous BMSCs indicated a beginning of bone healing as soon as 2 weeks with a complete healing after 9 weeks. At this stage, MRI and histological analysis were similar to a normal FH. Intra-osseous injection of BMSCs in FH seems to be a good strategy for ONFH treatment as the safety concerning the biodistribution of BMSCs is ensured. Moreover, the efficacy of BMSCs in natural ONFH seems a promising approach. Altogether, these results constitute the preclinical data necessary for the setup of a clinical application with expanded BMSCs in the context of Advanced Therapies Medicinal Products.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 3%
Unknown 57 97%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 15%
Student > Ph. D. Student 8 14%
Researcher 7 12%
Other 5 8%
Student > Doctoral Student 4 7%
Other 11 19%
Unknown 15 25%
Readers by discipline Count As %
Medicine and Dentistry 13 22%
Agricultural and Biological Sciences 9 15%
Biochemistry, Genetics and Molecular Biology 6 10%
Engineering 4 7%
Nursing and Health Professions 2 3%
Other 6 10%
Unknown 19 32%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2015.
All research outputs
#2,948,787
of 5,569,761 outputs
Outputs from Stem Cell Research & Therapy
#293
of 491 outputs
Outputs of similar age
#104,842
of 193,827 outputs
Outputs of similar age from Stem Cell Research & Therapy
#25
of 52 outputs
Altmetric has tracked 5,569,761 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 491 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 193,827 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.