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Exploiting single-molecule transcript sequencing for eukaryotic gene prediction

Overview of attention for article published in Genome Biology, September 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

news
3 news outlets
blogs
1 blog
twitter
29 X users

Citations

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127 Dimensions

Readers on

mendeley
179 Mendeley
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Title
Exploiting single-molecule transcript sequencing for eukaryotic gene prediction
Published in
Genome Biology, September 2015
DOI 10.1186/s13059-015-0729-7
Pubmed ID
Authors

André E. Minoche, Juliane C. Dohm, Jessica Schneider, Daniela Holtgräwe, Prisca Viehöver, Magda Montfort, Thomas Rosleff Sörensen, Bernd Weisshaar, Heinz Himmelbauer

Abstract

We develop a method to predict and validate gene models using PacBio single-molecule, real-time (SMRT) cDNA reads. Ninety-eight percent of full-insert SMRT reads span complete open reading frames. Gene model validation using SMRT reads is developed as automated process. Optimized training and prediction settings and mRNA-seq noise reduction of assisting Illumina reads results in increased gene prediction sensitivity and precision. Additionally, we present an improved gene set for sugar beet (Beta vulgaris) and the first genome-wide gene set for spinach (Spinacia oleracea). The workflow and guidelines are a valuable resource to obtain comprehensive gene sets for newly sequenced genomes of non-model eukaryotes.

X Demographics

X Demographics

The data shown below were collected from the profiles of 29 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 179 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 2%
Austria 2 1%
Spain 2 1%
Norway 1 <1%
Singapore 1 <1%
Germany 1 <1%
United Kingdom 1 <1%
Taiwan 1 <1%
Unknown 167 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 46 26%
Student > Ph. D. Student 40 22%
Student > Master 16 9%
Student > Bachelor 12 7%
Professor > Associate Professor 10 6%
Other 27 15%
Unknown 28 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 88 49%
Biochemistry, Genetics and Molecular Biology 36 20%
Computer Science 8 4%
Immunology and Microbiology 3 2%
Engineering 3 2%
Other 8 4%
Unknown 33 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 42. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2016.
All research outputs
#982,927
of 25,371,288 outputs
Outputs from Genome Biology
#692
of 4,467 outputs
Outputs of similar age
#13,242
of 277,045 outputs
Outputs of similar age from Genome Biology
#16
of 82 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,045 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 82 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.