Title |
Exploiting single-molecule transcript sequencing for eukaryotic gene prediction
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Published in |
Genome Biology, September 2015
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DOI | 10.1186/s13059-015-0729-7 |
Pubmed ID | |
Authors |
André E. Minoche, Juliane C. Dohm, Jessica Schneider, Daniela Holtgräwe, Prisca Viehöver, Magda Montfort, Thomas Rosleff Sörensen, Bernd Weisshaar, Heinz Himmelbauer |
Abstract |
We develop a method to predict and validate gene models using PacBio single-molecule, real-time (SMRT) cDNA reads. Ninety-eight percent of full-insert SMRT reads span complete open reading frames. Gene model validation using SMRT reads is developed as automated process. Optimized training and prediction settings and mRNA-seq noise reduction of assisting Illumina reads results in increased gene prediction sensitivity and precision. Additionally, we present an improved gene set for sugar beet (Beta vulgaris) and the first genome-wide gene set for spinach (Spinacia oleracea). The workflow and guidelines are a valuable resource to obtain comprehensive gene sets for newly sequenced genomes of non-model eukaryotes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 7 | 24% |
United States | 5 | 17% |
South Africa | 2 | 7% |
Belgium | 1 | 3% |
Australia | 1 | 3% |
Spain | 1 | 3% |
France | 1 | 3% |
Netherlands | 1 | 3% |
China | 1 | 3% |
Other | 1 | 3% |
Unknown | 8 | 28% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 15 | 52% |
Members of the public | 12 | 41% |
Science communicators (journalists, bloggers, editors) | 2 | 7% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 2% |
Austria | 2 | 1% |
Spain | 2 | 1% |
Norway | 1 | <1% |
Singapore | 1 | <1% |
Germany | 1 | <1% |
United Kingdom | 1 | <1% |
Taiwan | 1 | <1% |
Unknown | 167 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 46 | 26% |
Student > Ph. D. Student | 40 | 22% |
Student > Master | 16 | 9% |
Student > Bachelor | 12 | 7% |
Professor > Associate Professor | 10 | 6% |
Other | 27 | 15% |
Unknown | 28 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 88 | 49% |
Biochemistry, Genetics and Molecular Biology | 36 | 20% |
Computer Science | 8 | 4% |
Immunology and Microbiology | 3 | 2% |
Engineering | 3 | 2% |
Other | 8 | 4% |
Unknown | 33 | 18% |