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Eviction of linker histone H1 by NAP-family histone chaperones enhances activated transcription

Overview of attention for article published in Epigenetics & Chromatin, September 2015
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  • Good Attention Score compared to outputs of the same age (68th percentile)

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7 tweeters

Citations

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49 Mendeley
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Title
Eviction of linker histone H1 by NAP-family histone chaperones enhances activated transcription
Published in
Epigenetics & Chromatin, September 2015
DOI 10.1186/s13072-015-0022-8
Pubmed ID
Authors

Qian Zhang, Holli A. Giebler, Marisa K. Isaacson, Jennifer K. Nyborg

Abstract

In the Metazoan nucleus, core histones assemble the genomic DNA to form nucleosome arrays, which are further compacted into dense chromatin structures by the linker histone H1. The extraordinary density of chromatin creates an obstacle for accessing the genetic information. Regulation of chromatin dynamics is therefore critical to cellular homeostasis, and histone chaperones serve as prominent players in these processes. In the current study, we examined the role of specific histone chaperones in negotiating the inherently repressive chromatin structure during transcriptional activation. Using a model promoter, we demonstrate that the human nucleosome assembly protein family members hNap1 and SET/Taf1β stimulate transcription in vitro during pre-initiation complex formation, prior to elongation. This stimulatory effect is dependent upon the presence of activators, p300, and Acetyl-CoA. We show that transcription from our chromatin template is strongly repressed by H1, and that both histone chaperones enhance RNA synthesis by overcoming H1-induced repression. Importantly, both hNap1 and SET/Taf1β directly bind H1, and function to enhance transcription by evicting the linker histone from chromatin reconstituted with H1. In vivo studies demonstrate that SET/Taf1β, but not hNap1, strongly stimulates activated transcription from the chromosomally-integrated model promoter, consistent with the observation that SET/Taf1β is nuclear, whereas hNap1 is primarily cytoplasmic. Together, these observations indicate that SET/Taf1β may serve as a critical regulator of H1 dynamics and gene activation in vivo. These studies uncover a novel function for SET that mechanistically couples transcriptional derepression with H1 dynamics. Furthermore, they underscore the significance of chaperone-dependent H1 displacement as an essential early step in the transition of a promoter from a dense chromatin state into one that is permissive to transcription factor binding and robust activation.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
India 1 2%
South Africa 1 2%
Unknown 46 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 27%
Student > Master 8 16%
Student > Bachelor 7 14%
Student > Doctoral Student 5 10%
Researcher 5 10%
Other 3 6%
Unknown 8 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 41%
Biochemistry, Genetics and Molecular Biology 13 27%
Medicine and Dentistry 2 4%
Chemistry 2 4%
Social Sciences 1 2%
Other 3 6%
Unknown 8 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2015.
All research outputs
#5,096,436
of 16,530,923 outputs
Outputs from Epigenetics & Chromatin
#253
of 474 outputs
Outputs of similar age
#73,960
of 242,466 outputs
Outputs of similar age from Epigenetics & Chromatin
#1
of 1 outputs
Altmetric has tracked 16,530,923 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 474 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 242,466 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them