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Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis

Overview of attention for article published in Parasites & Vectors, September 2015
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  • Good Attention Score compared to outputs of the same age (75th percentile)

Mentioned by

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8 tweeters

Citations

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13 Dimensions

Readers on

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23 Mendeley
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Title
Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis
Published in
Parasites & Vectors, September 2015
DOI 10.1186/s13071-015-1048-2
Pubmed ID
Authors

Yuanbin Zhang, Jing Zhao, Xinye Wang, Xindong Xu, Weiqing Pan

Abstract

Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma japonicum infections. In this study, we evaluated these antigens as candidate biomarkers for the early diagnosis of schistosomiasis in mice and rabbits. The transcriptional profiles of the six antigens (SjSP-13, SjSP-23, SjSP-160, SjSP-164, SjSP-189 and SjSP-216) at different developmental stages were analyzed by quantitative PCR. The recombinant proteins were expressed in E. coli and purified with nickel chelate affinity chromatography. We then developed recombinant protein-based ELISA kits to analyze the kinetics of antigen-specific antibodies during the course of infection in mice and rabbits. The early diagnostic validity of the candidate SjSP-216 was further evaluated in mice and rabbits infected with S. japonicum. Of the six antigens, SjSP-13, SjSP-160 and SjSP-216 were highly expressed in 21-day old young worms, while SjSP-23, SjSP-164 and SjSP-189 were highly expressed in eggs. In the mouse model, we detected a significant increase in antibodies against SjSP-13 and SjSP-216 at 3 weeks post-infection. However, in the rabbit model, only anti-SjSP-216 antibody showed a significant increase at this time point. We recorded 100 % diagnostic sensitivity and specificity of SjSP-216-based ELISA in both infected mice and rabbits, 3 weeks after infection. This study strongly suggests that SjSP-216, a highly expressed gene in the young worms, could serve as a potential biomarker for the early immunodiagnosis of S. japonicum infections in vertebrate hosts.

Twitter Demographics

The data shown below were collected from the profiles of 8 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 35%
Researcher 7 30%
Student > Ph. D. Student 4 17%
Lecturer 1 4%
Student > Postgraduate 1 4%
Other 0 0%
Unknown 2 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 30%
Agricultural and Biological Sciences 4 17%
Medicine and Dentistry 4 17%
Nursing and Health Professions 2 9%
Immunology and Microbiology 2 9%
Other 1 4%
Unknown 3 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2016.
All research outputs
#4,270,415
of 16,518,952 outputs
Outputs from Parasites & Vectors
#899
of 4,395 outputs
Outputs of similar age
#60,024
of 242,862 outputs
Outputs of similar age from Parasites & Vectors
#1
of 1 outputs
Altmetric has tracked 16,518,952 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 4,395 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 242,862 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them