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Doublecortin-expressing cell types in temporal lobe epilepsy

Overview of attention for article published in Acta Neuropathologica Communications, July 2018
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Title
Doublecortin-expressing cell types in temporal lobe epilepsy
Published in
Acta Neuropathologica Communications, July 2018
DOI 10.1186/s40478-018-0566-5
Pubmed ID
Authors

Joan Y. W. Liu, Mar Matarin, Cheryl Reeves, Andrew W. McEvoy, Anna Miserocchi, Pamela Thompson, Sanjay M. Sisodiya, Maria Thom

Abstract

Doublecortin (DCX) is widely regarded as a marker of immature and migrating neurons during development. While DCX expression persists in adults, particularly in the temporal lobe and neurogenic regions, it is unknown how seizures influence its expression. The aim of the present study was to explore the distribution and characteristics of DCX-expressing cells in surgical and postmortem samples from 40 adult and paediatric patients, with epilepsy and with or without hippocampal sclerosis (HS), compared to post mortem controls. The hippocampus (pes and body), parahippocampal gyrus, amygdala, temporal pole and temporal cortex were examined with DCX immunohistochemistry using four commercially-available DCX antibodies, labelled cells were quantified in different regions of interest as well as their co-expression with cell type specific markers (CD68, Iba1, GFAP, GFAP∂, nestin, SOX2, CD34, OLIG2, PDGFRβ, NeuN) and cell cycle marker (MCM2). Histological findings were compared with clinical data, as well as gene expression data obtained from the temporal cortex of 83 temporal lobe epilepsy cases with HS. DCX immunohistochemistry identified immature (Nestin-/NeuN-) neurons in layer II of the temporal neocortex in patients with and without epilepsy. Their number declined significantly with age but was not associated with the presence of hippocampal sclerosis, seizure semiology or memory dysfunction. DCX+ cells were prominent in the paralaminar nuclei and periamygdalar cortex and these declined with age but were not significantly associated with epilepsy history. DCX expressing cells with ramified processes were prominent in all regions, particularly in the hippocampal subgranular zone, where significantly increased numbers were observed in epilepsy samples compared to controls. DCX ramified cells co-expressed Iba1, CD68 and PDGFRβ, and less frequently MCM2, OLIG2 and SOX2, but no co-localization was observed with CD34, nestin or GFAP/GFAP ∂. Gene expression data from neocortical samples in patients with TLE and HS supported ongoing DCX expression in adults. We conclude that DCX identifies a range of morphological cell types in temporal lobe epilepsy, including immature populations, glial and microglial cell types. Their clinical relevance and biological function requires further study but we show some evidence for alteration with age and in epilepsy.

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Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 49 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 18%
Researcher 9 18%
Student > Master 5 10%
Student > Bachelor 4 8%
Student > Postgraduate 3 6%
Other 5 10%
Unknown 14 29%
Readers by discipline Count As %
Neuroscience 18 37%
Biochemistry, Genetics and Molecular Biology 5 10%
Agricultural and Biological Sciences 2 4%
Social Sciences 2 4%
Psychology 2 4%
Other 4 8%
Unknown 16 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 July 2018.
All research outputs
#10,572,680
of 13,278,410 outputs
Outputs from Acta Neuropathologica Communications
#549
of 667 outputs
Outputs of similar age
#198,836
of 266,485 outputs
Outputs of similar age from Acta Neuropathologica Communications
#1
of 1 outputs
Altmetric has tracked 13,278,410 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 667 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.7. This one is in the 6th percentile – i.e., 6% of its peers scored the same or lower than it.
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