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Assessment of the in vitro growing dynamics and kinetics of the non-pathogenic J and pathogenic 11 and 232 Mycoplasma hyopneumoniae strains

Overview of attention for article published in Veterinary Research, May 2018
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Title
Assessment of the in vitro growing dynamics and kinetics of the non-pathogenic J and pathogenic 11 and 232 Mycoplasma hyopneumoniae strains
Published in
Veterinary Research, May 2018
DOI 10.1186/s13567-018-0541-y
Pubmed ID
Authors

Beatriz Garcia-Morante, Arkadius Dors, Rocio León-Kempis, Ana Pérez de Rozas, Joaquim Segalés, Marina Sibila

Abstract

Information on the in vitro growth of pathogenic and non-pathogenic Mycoplasma hyopneumoniae (M. hyopneumoniae) strains is scarce and controversial. Despite its limitations, the colour changing units (CCU) assay is still considered the golden standard titration technique for M. hyopneumoniae culture. Thus, the aims of the present study were: (1) to describe the growth dynamics and kinetics of pathogenic and non-pathogenic M. hyopneumoniae strains, and (2) to monitor the strains' daily growth by ATP luminometry, CCU, colony forming units (CFU), and DNA quantification by real time quantitative PCR (qPCR) and by fluorescent double-stranded DNA (dsDNA) staining, to evaluate them as putative titration methodologies. The growth of the non-pathogenic J (ATCC®25934™) type strain and the pathogenic 11 (ATCC®25095™) reference strain and 232 strain was modelled by the Gompertz model. Globally, all three-strain cultures showed the same growing phases as well as similar maximal titres within a particular technique, but for CFU. However, the J strain displayed the fastest growing. During the logarithmic phase of growing, CCU, ATP and M. hyopneumoniae copy titres were strongly and linearly associated, and correlation between techniques could be reliably established. In conclusion, real-time culture titration by means of ATP or molecular assays was useful to describe the in vitro growth of the tested strains. Knowledge about the in vitro growth behaviour of a specific strain in a specific medium may provide several advantages, including information about the time required to reach maximal titres by the culture. Noteworthy, the obtained results refers to the three strains used, so extrapolation to other M. hyopneumoniae strains or culture conditions should be made cautiously.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 33%
Student > Ph. D. Student 4 22%
Student > Postgraduate 2 11%
Student > Master 1 6%
Student > Bachelor 1 6%
Other 0 0%
Unknown 4 22%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 6 33%
Agricultural and Biological Sciences 2 11%
Nursing and Health Professions 1 6%
Biochemistry, Genetics and Molecular Biology 1 6%
Decision Sciences 1 6%
Other 1 6%
Unknown 6 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 July 2018.
All research outputs
#10,543,872
of 13,237,907 outputs
Outputs from Veterinary Research
#625
of 826 outputs
Outputs of similar age
#163,701
of 218,840 outputs
Outputs of similar age from Veterinary Research
#5
of 5 outputs
Altmetric has tracked 13,237,907 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 826 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
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