Title |
Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody
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Published in |
BMC Biotechnology, September 2015
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DOI | 10.1186/s12896-015-0201-5 |
Pubmed ID | |
Authors |
Shunli Yang, Shuanghui Yin, Youjun Shang, Di Wang, Weimin Ma, Jijun He, Jianhong Guo, Jianping Cai, Xiangtao Liu |
Abstract |
Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover, this method has been successfully used to capture Asia 1 antigen in synthetic samples. Ultimately, a specific and highly sensitive capture FMDV Asia 1 tool has been established that has the potential to enhance the sensitivity and reliability when diagnosing FMDV Asia 1. |
X Demographics
Geographical breakdown
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 20% |
Student > Master | 3 | 15% |
Student > Bachelor | 2 | 10% |
Student > Doctoral Student | 1 | 5% |
Student > Ph. D. Student | 1 | 5% |
Other | 0 | 0% |
Unknown | 9 | 45% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Veterinary Science and Veterinary Medicine | 3 | 15% |
Immunology and Microbiology | 2 | 10% |
Business, Management and Accounting | 1 | 5% |
Unknown | 9 | 45% |