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Tissue-specific expression of histone H3 variants diversified after species separation

Overview of attention for article published in Epigenetics & Chromatin, September 2015
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  • Good Attention Score compared to outputs of the same age (73rd percentile)

Mentioned by

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10 tweeters

Citations

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39 Dimensions

Readers on

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62 Mendeley
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Title
Tissue-specific expression of histone H3 variants diversified after species separation
Published in
Epigenetics & Chromatin, September 2015
DOI 10.1186/s13072-015-0027-3
Pubmed ID
Authors

Kazumitsu Maehara, Akihito Harada, Yuko Sato, Masaki Matsumoto, Keiichi I. Nakayama, Hiroshi Kimura, Yasuyuki Ohkawa

Abstract

The selective incorporation of appropriate histone variants into chromatin is critical for the regulation of genome function. Although many histone variants have been identified, a complete list has not been compiled. We screened mouse, rat and human genomes by in silico hybridization using canonical histone sequences. In the mouse genome, we identified 14 uncharacterized H3 genes, among which 13 are similar to H3.3 and do not have human or rat counterparts, and one is similar to human testis-specific H3 variant, H3T/H3.4, and had a rat paralog. Although some of these genes were previously annotated as pseudogenes, their tissue-specific expression was confirmed by sequencing the 3'-UTR regions of the transcripts. Certain new variants were also detected at the protein level by mass spectrometry. When expressed as GFP-tagged versions in mouse C2C12 cells, some variants were stably incorporated into chromatin and the genome-wide distributions of most variants were similar to that of H3.3. Moreover, forced expression of H3 variants in chromatin resulted in alternate gene expression patterns after cell differentiation. We comprehensively identified and characterized novel mouse H3 variant genes that encoded highly conserved amino acid sequences compared to known histone H3. We speculated that the diversity of H3 variants acquired after species separation played a role in regulating tissue-specific gene expression in individual species. Their biological relevance and evolutionary aspect involving pseudogene diversification will be addressed by further functional analysis.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 61 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 21%
Researcher 11 18%
Student > Master 7 11%
Student > Bachelor 5 8%
Student > Doctoral Student 5 8%
Other 10 16%
Unknown 11 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 30 48%
Agricultural and Biological Sciences 12 19%
Immunology and Microbiology 2 3%
Medicine and Dentistry 2 3%
Computer Science 1 2%
Other 5 8%
Unknown 10 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 October 2015.
All research outputs
#5,442,644
of 20,830,231 outputs
Outputs from Epigenetics & Chromatin
#221
of 538 outputs
Outputs of similar age
#67,402
of 260,350 outputs
Outputs of similar age from Epigenetics & Chromatin
#1
of 1 outputs
Altmetric has tracked 20,830,231 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 538 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 260,350 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them