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Phosphoproteome dynamics mediate revival of bacterial spores

Overview of attention for article published in BMC Biology, September 2015
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Title
Phosphoproteome dynamics mediate revival of bacterial spores
Published in
BMC Biology, September 2015
DOI 10.1186/s12915-015-0184-7
Pubmed ID
Authors

Alex Rosenberg, Boumediene Soufi, Vaishnavi Ravikumar, Nelson C. Soares, Karsten Krug, Yoav Smith, Boris Macek, Sigal Ben-Yehuda

Abstract

Bacterial spores can remain dormant for decades, yet harbor the exceptional capacity to rapidly resume metabolic activity and recommence life. Although germinants and their corresponding receptors have been known for more than 30 years, the molecular events underlying this remarkable cellular transition from dormancy to full metabolic activity are only partially defined. Here, we examined whether protein phospho-modifications occur during germination, the first step of exiting dormancy, thereby facilitating spore revival. Utilizing Bacillus subtilis as a model organism, we performed phosphoproteomic analysis to define the Ser/Thr/Tyr phosphoproteome of a reviving spore. The phosphoproteome was found to chiefly comprise newly identified phosphorylation sites located within proteins involved in basic biological functions, such as transcription, translation, carbon metabolism, and spore-specific determinants. Quantitative comparison of dormant and germinating spore phosphoproteomes revealed phosphorylation dynamics, indicating that phospho-modifications could modulate protein activity during this cellular transition. Furthermore, by mutating select phosphorylation sites located within proteins representative of key biological processes, we established a functional connection between phosphorylation and the progression of spore revival. Herein, we provide, for the first time, a phosphoproteomic view of a germinating bacterial spore. We further show that the spore phosphoproteome is dynamic and present evidence that phosphorylation events play an integral role in facilitating spore revival.

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The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Unknown 52 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 19%
Researcher 9 17%
Student > Bachelor 7 13%
Student > Postgraduate 5 9%
Student > Master 4 8%
Other 7 13%
Unknown 11 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 28%
Biochemistry, Genetics and Molecular Biology 13 25%
Engineering 3 6%
Immunology and Microbiology 3 6%
Chemistry 2 4%
Other 3 6%
Unknown 14 26%