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Highly discordant serology against Trypanosoma cruzi in central Veracruz, Mexico: role of the antigen used for diagnostic

Overview of attention for article published in Parasites & Vectors, September 2015
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Title
Highly discordant serology against Trypanosoma cruzi in central Veracruz, Mexico: role of the antigen used for diagnostic
Published in
Parasites & Vectors, September 2015
DOI 10.1186/s13071-015-1072-2
Pubmed ID
Authors

Daniel Guzmán-Gómez, Aracely López-Monteon, María de la Soledad Lagunes-Castro, Carolina Álvarez-Martínez, Manuel Jesús Hernández-Lutzon, Eric Dumonteil, Angel Ramos-Ligonio

Abstract

Chagas disease is a parasitic disease caused by the protozoan parasite Trypanosoma cruzi. In Mexico, the burden of the disease is difficult to estimate and improving surveillance for Chagas disease is an important priority. We aimed here at determining the seroprevalence of T. cruzi infection in humans in a rural community in Veracruz. Serum samples (196) were analyzed for T. cruzi infection using five enzyme-linked immunosorbent assay (ELISA) tests: two in-house tests based on crude parasite extract and three commercial ELISA kits. Because of highly discordant results, we further explored the importance of parasite antigens and strains by western-blot analysis. A total of 74 samples (37.7 %) were reactive with at least one ELISA, but discordance among tests was very high. The best agreement was between Chagatest recombinant and Chagatek ELISA (Kappa index = 0.798). The agreement between other combinations of tests ranged from 0.038 to 0.518. Discordant samples were confirmed by western-blot analysis using up to nine parasite strains, giving a seroprevalence of 33.7 %. Commercial tests had a very limited ability to detect T. cruzi infection in the study population. In-house tests based on crude parasite antigens showed a greater sensitivity but were still unable to detect all cases of T. cruzi infection, even when based on a local parasite strain. The high seroprevalence confirmed the hyper-endemicity of T. cruzi infection in the region. Reliable epidemiological surveillance of Chagas disease will require the development of improved diagnostic tests.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 1%
Germany 1 1%
Unknown 95 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 21 22%
Researcher 15 15%
Student > Ph. D. Student 10 10%
Student > Bachelor 10 10%
Student > Doctoral Student 6 6%
Other 9 9%
Unknown 26 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 16%
Medicine and Dentistry 13 13%
Biochemistry, Genetics and Molecular Biology 13 13%
Immunology and Microbiology 9 9%
Pharmacology, Toxicology and Pharmaceutical Science 3 3%
Other 10 10%
Unknown 33 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2015.
All research outputs
#20,291,881
of 22,828,180 outputs
Outputs from Parasites & Vectors
#4,846
of 5,463 outputs
Outputs of similar age
#228,664
of 272,396 outputs
Outputs of similar age from Parasites & Vectors
#125
of 146 outputs
Altmetric has tracked 22,828,180 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,463 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 146 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.