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Molecular and Phylogenetic analysis revealed new genotypes of Theileria annulata parasites from India

Overview of attention for article published in Parasites & Vectors, September 2015
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  • Above-average Attention Score compared to outputs of the same age (51st percentile)
  • Above-average Attention Score compared to outputs of the same age and source (62nd percentile)

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Title
Molecular and Phylogenetic analysis revealed new genotypes of Theileria annulata parasites from India
Published in
Parasites & Vectors, September 2015
DOI 10.1186/s13071-015-1075-z
Pubmed ID
Authors

Neena George, Vasundhra Bhandari, D. Peddi Reddy, Paresh Sharma

Abstract

Tick borne diseases impinge cattle worldwide causing mortality and resulting in huge economic losses. Theileriosis is one of the important tick borne diseases mainly caused by Theileria annulata and one of the commonly occurring infections among the livestock. T. annulata causes immense loss to the livestock industry and therefore, efficacious eradication and control strategies are needed for the control of the disease. Genetic diversity among T. annulata parasites is another important aspect which is overlooked in India. Thus, the present study aims to evaluate the prevalence along with genetic diversity and phylogeny of the prevailing T. annulata population of India. Genomic DNA was extracted from cattle blood samples (n = 862) from different regions of Andhra Pradesh. Molecular diagnosis using T. annulata 18S rRNA based PCR was performed to detect parasites in cattle. Further, 18S rRNA gene was cloned and sequenced to determine similarity and diversity from the known T. annulata sequences. We observed an overall prevalence rate of 32.40 % T. annulata infection in Andhra Pradesh based on PCR assay. The sequence analysis revealed novel genotypes among the T. annulata strains from India. Thirteen strains showed closed proximity with a strain from China whereas one Indian strain showed similarity with a South African strain [Theileria sp (buffalo)] based on phylogenetic analysis. Nucleotide heterogeneity of the 18S rRNA sequence among the strains examined varied from 0.1 to 8.6 % when compared with the published strains. The present study provides us with the molecular prevalence of theileriosis, and will support the accomplishment of actions or in design of strategy to control theileriosis transmission to cattle. Additionally, it highlights the emergence of strains with novel genotypes from India.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 48 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 20%
Student > Ph. D. Student 9 18%
Student > Postgraduate 5 10%
Student > Doctoral Student 4 8%
Student > Master 4 8%
Other 10 20%
Unknown 7 14%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 13 27%
Agricultural and Biological Sciences 11 22%
Biochemistry, Genetics and Molecular Biology 11 22%
Immunology and Microbiology 2 4%
Arts and Humanities 1 2%
Other 1 2%
Unknown 10 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2015.
All research outputs
#13,447,737
of 22,829,083 outputs
Outputs from Parasites & Vectors
#2,455
of 5,463 outputs
Outputs of similar age
#128,521
of 272,396 outputs
Outputs of similar age from Parasites & Vectors
#52
of 146 outputs
Altmetric has tracked 22,829,083 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,463 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 272,396 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 146 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.