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Gene expression changes in the salivary glands of Anopheles coluzzii elicited by Plasmodium berghei infection

Overview of attention for article published in Parasites & Vectors, September 2015
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Title
Gene expression changes in the salivary glands of Anopheles coluzzii elicited by Plasmodium berghei infection
Published in
Parasites & Vectors, September 2015
DOI 10.1186/s13071-015-1079-8
Pubmed ID
Authors

Renato Pinheiro-Silva, Lara Borges, Luís Pedro Coelho, Alejandro Cabezas-Cruz, James J. Valdés, Virgílio do Rosário, José de la Fuente, Ana Domingos

Abstract

Malaria is a devastating infectious disease caused by Plasmodium parasites transmitted through the bites of infected Anopheles mosquitoes. Salivary glands are the only mosquito tissue invaded by Plasmodium sporozoites, being a key stage for the effective parasite transmission, making the study of Anopheles sialome highly relevant. RNA-sequencing was used to compare differential gene expression in salivary glands of uninfected and Plasmodium berghei-infected Anopheles coluzzii mosquitoes. RNA-seq results were validated by quantitative RT-PCR. The transmembrane glucose transporter gene AGAP007752 was selected for functional analysis by RNA interference. The effect of gene silencing on infection level was evaluated. The putative function and tertiary structure of the protein was assessed. RNA-seq data showed that 2588 genes were differentially expressed in mosquitoes salivary glands in response to P. berghei infection, being 1578 upregulated and 1010 downregulated. Metabolism, Immunity, Replication/Transcription/Translation, Proteolysis and Transport were the mosquito gene functional classes more affected by parasite infection. Endopeptidase coding genes were the most abundant within the differentially expressed genes in infected salivary glands (P < 0.001). Based on its putative function and expression level, the transmembrane glucose transporter gene, AGAP007752, was selected for functional analysis by RNA interference. The results demonstrated that the number of sporozoites was 44.3 % lower in mosquitoes fed on infected mice after AGAPP007752 gene knockdown when compared to control (P < 0.01). Our hypothesis is that the protein encoded by the gene AGAPP007752 may play a role on An. coluzzii salivary glands infection by Plasmodium parasite, working as a sporozoite receptor and/or promoting a favorable environment for the capacity of sporozoites.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Germany 1 2%
Ghana 1 2%
Unknown 61 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 25%
Student > Master 13 20%
Researcher 10 16%
Student > Bachelor 5 8%
Student > Postgraduate 2 3%
Other 5 8%
Unknown 13 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 21 33%
Biochemistry, Genetics and Molecular Biology 14 22%
Veterinary Science and Veterinary Medicine 3 5%
Medicine and Dentistry 3 5%
Immunology and Microbiology 2 3%
Other 6 9%
Unknown 15 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2015.
All research outputs
#14,825,907
of 22,829,083 outputs
Outputs from Parasites & Vectors
#3,082
of 5,463 outputs
Outputs of similar age
#151,658
of 274,809 outputs
Outputs of similar age from Parasites & Vectors
#71
of 147 outputs
Altmetric has tracked 22,829,083 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,463 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 274,809 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 147 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.