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Does adipose tissue-derived stem cell therapy improve graft quality in freshly grafted ovaries?

Overview of attention for article published in Reproductive Biology and Endocrinology, September 2015
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2 tweeters

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22 Mendeley
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Title
Does adipose tissue-derived stem cell therapy improve graft quality in freshly grafted ovaries?
Published in
Reproductive Biology and Endocrinology, September 2015
DOI 10.1186/s12958-015-0104-2
Pubmed ID
Authors

Luciana L. Damous, Juliana S. Nakamuta, Ana ET Saturi de Carvalho, Katia Candido Carvalho, José Maria Soares-Jr, Manuel de Jesus Simões, José Eduardo Krieger, Edmund Chada Baracat

Abstract

A major concern in ovarian transplants is substantial follicle loss during the initial period of hypoxia. Adipose tissue-derived stem cells (ASCs) have been employed to improve angiogenesis when injected into ischemic tissue. This study evaluated the safety and efficacy of adipose tissue-derived stem cells (ASCs) therapy in the freshly grafted ovaries 30 days after injection. Rat ASCs (rASCs) obtained from transgenic rats expressing green fluorescent protein (GFP)-(5 × 10(4) cells/ovary) were injected in topic (intact) or freshly grafted ovaries of 30 twelve-week-old adult female Wistar rats. The whole ovary was grafted in the retroperitoneum without vascular anastomosis, immediately after oophorectomy. Vaginal smears were performed daily to assess the resumption of the estrous cycle. Estradiol levels, grafts morphology and follicular viability and density were analyzed. Immunohistochemistry assays were conducted to identify and quantify rASC-GFP(+), VEGF tissue expression, apoptosis (cleaved caspase-3 and TUNEL), and cell proliferation (Ki-67). Quantitative gene expression (qPCR) for VEGF-A, Bcl2, EGF and TGF-β1 was evaluated using RT-PCR and a double labeling immunofluorescence assay for GFP and Von Willebrand Factor (VWF) was performed. Grafted ovaries treated with rASC-GFP(+) exhibited earlier resumption of the estrous phase (p < 0.05), increased VEGF-A expression (11-fold in grafted ovaries and 5-fold in topic ovaries vs. control) and an increased number of blood vessels (p < 0.05) in ovarian tissue without leading to apoptosis or cellular proliferation (p > 0.05). Estradiol levels were similar among groups (p > 0.05). rASC-GFP(+) were observed in similar quantities in the topic and grafted ovaries (p > 0.05), and double-labeling for GFP and vWF was observed in both injected groups. rASC therapy in autologous freshly ovarian grafts could be feasible and safe, induces earlier resumption of the estrous phase and enhances blood vessels in rats. This pilot study may be useful in the future for new researches on frozen-thawed ovarian tissue.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 5%
Unknown 21 95%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 14%
Other 2 9%
Researcher 2 9%
Student > Ph. D. Student 2 9%
Student > Bachelor 2 9%
Other 3 14%
Unknown 8 36%
Readers by discipline Count As %
Medicine and Dentistry 5 23%
Engineering 2 9%
Agricultural and Biological Sciences 2 9%
Immunology and Microbiology 1 5%
Biochemistry, Genetics and Molecular Biology 1 5%
Other 2 9%
Unknown 9 41%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2015.
All research outputs
#4,725,328
of 6,395,401 outputs
Outputs from Reproductive Biology and Endocrinology
#199
of 319 outputs
Outputs of similar age
#137,973
of 200,749 outputs
Outputs of similar age from Reproductive Biology and Endocrinology
#6
of 14 outputs
Altmetric has tracked 6,395,401 research outputs across all sources so far. This one is in the 14th percentile – i.e., 14% of other outputs scored the same or lower than it.
So far Altmetric has tracked 319 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 200,749 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.