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The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, July 2018
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (72nd percentile)
  • Good Attention Score compared to outputs of the same age and source (78th percentile)

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Title
The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail
Published in
Biotechnology for Biofuels and Bioproducts, July 2018
DOI 10.1186/s13068-018-1199-4
Pubmed ID
Authors

Gerdt Müller, Piotr Chylenski, Bastien Bissaro, Vincent G. H. Eijsink, Svein Jarle Horn

Abstract

The discovery of enzymes named lytic polysaccharide monooxygenases (LPMOs) has had a major impact on the efficiency of current commercial cellulase cocktails for saccharification of lignocellulosic biomass. However, the notion that LPMOs use molecular oxygen as a co-substrate and require two externally delivered electrons per catalytic cycle poses a challenge in the development of efficient large-scale industrial processes. Building on the recent discovery that H2O2, rather than O2, is the co-substrate of LPMOs, we show here how cellulose degradation by the LPMO-containing commercial cellulase cocktail Cellic® CTec2 can be controlled and boosted by supplying the reaction with H2O2. The controlled supply of anaerobic hydrolysis reactions with H2O2 and sub-stoichiometric amounts of reductant increased apparent LPMO activity by almost two orders of magnitude compared to standard aerobic reactions utilizing O2 and stoichiometric amounts of reductant. Improved LPMO activity was correlated with enhanced saccharification rates and yields for a model cellulosic substrate (Avicel) as well as industrial lignocellulosic substrates (sulfite-pulped Norway spruce and steam-exploded birch), although the magnitude of the effects was substrate dependent. Improvements in lignocellulose conversions were achieved at low H2O2 feeding rates (in the range of 90-600 µM h-1). Tight control of LPMO reactions by controlled supply of H2O2 under anaerobic conditions was possible. We report saccharification rates and yields for a model substrate (Avicel) and industrial lignocellulosic substrates that, at low H2O2 feeding rates, are higher than those seen under standard aerobic conditions. In an industrial setting, controlling and supplying molecular oxygen and stoichiometric amounts of reductant are challenging. The present report shows that the use of small amounts of a liquid bulk chemical, H2O2, provides an alternative to the currently available processes, which likely is cheaper and more easy to control, while giving higher product yields.

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The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 197 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 197 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 39 20%
Student > Master 26 13%
Researcher 25 13%
Student > Bachelor 18 9%
Professor 9 5%
Other 30 15%
Unknown 50 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 61 31%
Agricultural and Biological Sciences 23 12%
Chemistry 16 8%
Chemical Engineering 8 4%
Engineering 6 3%
Other 16 8%
Unknown 67 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 August 2018.
All research outputs
#5,311,777
of 25,385,509 outputs
Outputs from Biotechnology for Biofuels and Bioproducts
#310
of 1,578 outputs
Outputs of similar age
#93,323
of 340,712 outputs
Outputs of similar age from Biotechnology for Biofuels and Bioproducts
#10
of 47 outputs
Altmetric has tracked 25,385,509 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,578 research outputs from this source. They receive a mean Attention Score of 4.9. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 340,712 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.