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The elongation factor eEF3 (Yef3) interacts with mRNA in a translation independent manner

Overview of attention for article published in BMC Molecular Biology, September 2015
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Title
The elongation factor eEF3 (Yef3) interacts with mRNA in a translation independent manner
Published in
BMC Molecular Biology, September 2015
DOI 10.1186/s12867-015-0045-5
Pubmed ID
Authors

Nitzan Samra, Avigail Atir-Lande, Lilach Pnueli, Yoav Arava

Abstract

mRNA binding proteins (RBPs) constitute 10-15 % of the eukaryotic proteome and play important part in post-transcriptional regulation of gene expression. Due to the instability of RNA and the transient nature its interaction with RBPs, identification of novel RBPs is a significant challenge. Recently, a novel methodology for RBP purification and identification (termed RaPID) was presented, which allows high affinity purification of RBPs while associated with mRNA in vivo. We performed a RaPID screen for proteins that interact with PMP1 mRNA in order to identify novel mRNA binding proteins. PMP1 mRNA was tagged in its 3' UTR with multiple MS2 loops and co-expressed with MS2-binding protein fused to streptavidin binding protein (SBP). RNA-protein complexes were cross-linked in vivo and isolated through streptavidin beads. The eluted proteins were subjected to mass spectroscopy analysis. The screen identified many proteins, about half of them were previously shown to bind RNA. We focused on eEF3 (YEF3), an essential translation elongation factor that interacts with ribosomes. Purification of TAP-tagged Yef3 with its associated RNAs confirmed that the native PMP1 transcript is associated with it. Intriguingly, high association with Yef3-TAP was observed when purification was performed in the presence of EDTA, and with PMP1 that contains stop codons immediately downstream to the initiation codon. Furthermore, high association was observed with a transcript containing only the 3' UTR of PMP1. Complementary, RaPID isolation of MS2-tagged 3' UTRs with their associated proteins revealed that Yef3 can efficiently interact with these regions. This study identifies many novel proteins that interact with PMP1 mRNA. Importantly, the elongation factor Yef3 was found to interact with mRNA in non-coding regions and in a translation independent manner. These results suggest an additional, non-elongation function for this factor.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Israel 1 4%
Canada 1 4%
Unknown 22 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 46%
Student > Doctoral Student 2 8%
Student > Master 2 8%
Student > Bachelor 2 8%
Researcher 2 8%
Other 2 8%
Unknown 3 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 46%
Biochemistry, Genetics and Molecular Biology 7 29%
Medicine and Dentistry 2 8%
Chemistry 1 4%
Unknown 3 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2016.
All research outputs
#6,202,333
of 8,166,192 outputs
Outputs from BMC Molecular Biology
#120
of 177 outputs
Outputs of similar age
#164,475
of 238,028 outputs
Outputs of similar age from BMC Molecular Biology
#4
of 4 outputs
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