↓ Skip to main content

The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages

Overview of attention for article published in Genome Biology, September 2015
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (69th percentile)

Mentioned by

9 tweeters


53 Dimensions

Readers on

101 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages
Published in
Genome Biology, September 2015
DOI 10.1186/s13059-015-0771-5
Pubmed ID

Shruthi Sridhar Vembar, Cameron Ross Macpherson, Odile Sismeiro, Jean-Yves Coppée, Artur Scherf


Transcriptome-wide ribosome occupancy studies have suggested that during the intra-erythrocytic lifecycle of Plasmodium falciparum, select mRNAs are post-transcriptionally regulated. A subset of these encodes parasite virulence factors required for invading host erythrocytes, and are currently being developed as vaccine candidates. However, the molecular mechanisms that govern post-transcriptional regulation are currently unknown. We explore the previously identified DNA/RNA-binding protein PfAlba1, which localizes to multiple foci in the cytoplasm of P. falciparum trophozoites. We establish that PfAlba1 is essential for asexual proliferation, and subsequently investigate parasites overexpressing epitope-tagged PfAlba1 to identify its RNA targets and effects on mRNA homeostasis and translational regulation. Using deep sequencing of affinity-purified PfAlba1-associated RNAs, we identify 1193 transcripts that directly bind to PfAlba1 in trophozoites. For 105 such transcripts, 43 % of which are uncharacterized and 13 % of which encode erythrocyte invasion components, the steady state levels significantly change at this stage, evidencing a role for PfAlba1 in maintaining mRNA homeostasis. Additionally, we discover that binding of PfAlba1 to four erythrocyte invasion mRNAs, Rap1, RhopH3, CDPK1, and AMA1, is linked to translation repression in trophozoites whereas release of these mRNAs from a PfAlba1 complex in mature stages correlates with protein synthesis. We show that PfAlba1 binds to a sub-population of asexual stage mRNAs and fine-tunes the timing of translation. This mode of post-transcriptional regulation may be especially important for P. falciparum erythrocyte invasion components that have to be assembled into apical secretory organelles in a highly time-dependent manner towards the end of the parasite's asexual lifecycle.

Twitter Demographics

Twitter Demographics

The data shown below were collected from the profiles of 9 tweeters who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 101 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Nigeria 1 <1%
Unknown 100 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 34 34%
Student > Master 12 12%
Researcher 11 11%
Student > Bachelor 7 7%
Student > Doctoral Student 6 6%
Other 13 13%
Unknown 18 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 31 31%
Agricultural and Biological Sciences 30 30%
Medicine and Dentistry 6 6%
Computer Science 5 5%
Immunology and Microbiology 2 2%
Other 4 4%
Unknown 23 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 October 2015.
All research outputs
of 24,003,070 outputs
Outputs from Genome Biology
of 4,279 outputs
Outputs of similar age
of 277,888 outputs
Outputs of similar age from Genome Biology
of 87 outputs
Altmetric has tracked 24,003,070 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 4,279 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.9. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,888 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 87 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.