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The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages

Overview of attention for article published in Genome Biology (Online Edition), September 2015
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  • Good Attention Score compared to outputs of the same age (72nd percentile)
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Mentioned by

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9 tweeters

Citations

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47 Dimensions

Readers on

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95 Mendeley
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Title
The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages
Published in
Genome Biology (Online Edition), September 2015
DOI 10.1186/s13059-015-0771-5
Pubmed ID
Authors

Shruthi Sridhar Vembar, Cameron Ross Macpherson, Odile Sismeiro, Jean-Yves Coppée, Artur Scherf

Abstract

Transcriptome-wide ribosome occupancy studies have suggested that during the intra-erythrocytic lifecycle of Plasmodium falciparum, select mRNAs are post-transcriptionally regulated. A subset of these encodes parasite virulence factors required for invading host erythrocytes, and are currently being developed as vaccine candidates. However, the molecular mechanisms that govern post-transcriptional regulation are currently unknown. We explore the previously identified DNA/RNA-binding protein PfAlba1, which localizes to multiple foci in the cytoplasm of P. falciparum trophozoites. We establish that PfAlba1 is essential for asexual proliferation, and subsequently investigate parasites overexpressing epitope-tagged PfAlba1 to identify its RNA targets and effects on mRNA homeostasis and translational regulation. Using deep sequencing of affinity-purified PfAlba1-associated RNAs, we identify 1193 transcripts that directly bind to PfAlba1 in trophozoites. For 105 such transcripts, 43 % of which are uncharacterized and 13 % of which encode erythrocyte invasion components, the steady state levels significantly change at this stage, evidencing a role for PfAlba1 in maintaining mRNA homeostasis. Additionally, we discover that binding of PfAlba1 to four erythrocyte invasion mRNAs, Rap1, RhopH3, CDPK1, and AMA1, is linked to translation repression in trophozoites whereas release of these mRNAs from a PfAlba1 complex in mature stages correlates with protein synthesis. We show that PfAlba1 binds to a sub-population of asexual stage mRNAs and fine-tunes the timing of translation. This mode of post-transcriptional regulation may be especially important for P. falciparum erythrocyte invasion components that have to be assembled into apical secretory organelles in a highly time-dependent manner towards the end of the parasite's asexual lifecycle.

Twitter Demographics

The data shown below were collected from the profiles of 9 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 95 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Nigeria 1 1%
Unknown 94 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 33 35%
Researcher 13 14%
Student > Master 12 13%
Student > Bachelor 7 7%
Student > Doctoral Student 6 6%
Other 11 12%
Unknown 13 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 35%
Biochemistry, Genetics and Molecular Biology 30 32%
Medicine and Dentistry 6 6%
Computer Science 4 4%
Immunology and Microbiology 2 2%
Other 3 3%
Unknown 17 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 October 2015.
All research outputs
#3,309,196
of 12,486,858 outputs
Outputs from Genome Biology (Online Edition)
#1,956
of 2,835 outputs
Outputs of similar age
#66,127
of 246,883 outputs
Outputs of similar age from Genome Biology (Online Edition)
#2
of 7 outputs
Altmetric has tracked 12,486,858 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 2,835 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 22.9. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 246,883 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.