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In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte-adapted P. knowlesi A1-H.1 strain

Overview of attention for article published in Malaria Journal, July 2018
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  • Above-average Attention Score compared to outputs of the same age (56th percentile)

Mentioned by

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5 tweeters

Citations

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7 Dimensions

Readers on

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42 Mendeley
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Title
In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte-adapted P. knowlesi A1-H.1 strain
Published in
Malaria Journal, July 2018
DOI 10.1186/s12936-018-2420-4
Pubmed ID
Authors

Fauzi Muh, Seong-Kyun Lee, Mohammad Rafiul Hoque, Jin-Hee Han, Ji-Hoon Park, Egy Rahman Firdaus, Robert W. Moon, Yee Ling Lau, Eun-Taek Han

Abstract

The rapid process of malaria erythrocyte invasion involves ligand-receptor interactions. Inducing antibodies against specific ligands or receptors that abrogate the invasion process is a key challenge for blood stage vaccine development. However, few candidates were reported and remain to be validated for the discovery of new vaccine candidates in Plasmodium knowlesi. In order to investigate the efficacy of pre-clinical vaccine candidates in P. knowlesi-infected human cases, this study describes an in vitro invasion inhibition assay, using a P. knowlesi strain adapted to in vitro growth in human erythrocytes, PkA1-H.1. Recombinant proteins of P. knowlesi Duffy binding protein alpha (PkDBPα) and apical membrane antigen 1 (PkAMA1) were produced in Escherichia coli system and rabbit antibodies were generated from immune animals. PkDBPα and PkAMA1 recombinant proteins were expressed as insoluble and produced as a functional refolded form for this study. Antibodies against PkDBPα and PkAMA1 specifically recognized recombinant proteins and native parasite proteins in schizont-stage parasites on the merozoite organelles. Single and combination of anti-PkDBPα and anti-PkAMA1 antibodies elicited strong growth inhibitory effects on the parasite in concentration-dependent manner. Meanwhile, IgG prevalence of PkDBPα and PkAMA1 were observed in 13.0 and 46.7% in human clinical patients, respectively. These data provide support for the validation of in vitro growth inhibition assay using antibodies of DBPα and AMA1 in human-adapted P. knowlesi parasite PkA1-H.1 strain.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 19%
Researcher 6 14%
Student > Bachelor 6 14%
Student > Master 5 12%
Lecturer 2 5%
Other 7 17%
Unknown 8 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 26%
Medicine and Dentistry 7 17%
Agricultural and Biological Sciences 4 10%
Immunology and Microbiology 3 7%
Business, Management and Accounting 1 2%
Other 6 14%
Unknown 10 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 August 2018.
All research outputs
#6,984,036
of 13,316,854 outputs
Outputs from Malaria Journal
#2,111
of 3,889 outputs
Outputs of similar age
#115,524
of 268,524 outputs
Outputs of similar age from Malaria Journal
#1
of 1 outputs
Altmetric has tracked 13,316,854 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,889 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,524 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them