Title |
Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells
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Published in |
BMC Plant Biology, September 2015
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DOI | 10.1186/s12870-015-0601-7 |
Pubmed ID | |
Authors |
Nicole Gaude, Silvia Bortfeld, Alexander Erban, Joachim Kopka, Franziska Krajinski |
Abstract |
The arbuscular mycorrhizal symbiosis is characterized by the presence of different symbiotic structures and stages within a root system. Therefore tools allowing the analysis of molecular changes at a cellular level are required to reveal insight into arbuscular mycorrhizal (AM) symbiosis development and functioning. Here we describe the analysis of metabolite pools in arbuscule-containing cells, which are the site of nutrient transfer between AM fungus and host plant. Laser capture microdissection (LCM) combined with gas chromatography mass spectrometry (GC-EI/TOF-MS) enabled the analysis of primary metabolite levels,which might be of plant or fungal origin, within these cells. High levels of the amino acids, aspartate, asparagine, glutamate, and glutamine, were observed in arbuscule-containing cells. Elevated amounts of sucrose and the steady-state of hexose levels indicated a direct assimilation of monosaccharides by the fungal partner. |
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Demographic breakdown
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Members of the public | 2 | 100% |
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Demographic breakdown
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Student > Ph. D. Student | 10 | 22% |
Student > Bachelor | 7 | 15% |
Student > Master | 6 | 13% |
Professor > Associate Professor | 4 | 9% |
Student > Doctoral Student | 3 | 7% |
Other | 7 | 15% |
Unknown | 9 | 20% |
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Nursing and Health Professions | 1 | 2% |
Other | 3 | 7% |
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