Title |
Pathogenesis and phylogenetic analyses of canine distemper virus strain ZJ7 isolate from domestic dogs in China
|
---|---|
Published in |
Virology Journal, November 2011
|
DOI | 10.1186/1743-422x-8-520 |
Pubmed ID | |
Authors |
Bin Tan, Yong-Jun Wen, Feng-Xue Wang, Shu-Qin Zhang, Xiu-Dong Wang, Jia-Xin Hu, Xin-Chuan Shi, Bo-Chao Yang, Li-Zhi Chen, Shi-Peng Cheng, Hua Wu |
Abstract |
A new isolate of canine distemper virus (CDV), named ZJ7, was isolated from lung tissues of a dog suspected with CDV infection using MDCK cells. The ZJ7 isolate induced cytopathogenic effects of syncytia in MDCK cell after six passages. In order to evaluate pathogenesis of ZJ7 strain, three CDV sero-negative dogs were intranasally inoculated with its virus suspension. All infected dogs developed clinical signs of severe bloody diarrhea, conjunctivitis, ocular discharge, nasal discharge and coughing, fever and weight loss at 21 dpi, whereas the mock group infected with DMEM were normal. The results demonstrated that CDV-ZJ7 strain isolated by MDCK cell was virulent, and the nucleotide and amino acid sequences of strain ZJ7 had no change after isolation by MDCK cell when compared with the original virus from the fresh tissues. Molecular and phylogenetic analyses for the nucleocapsid (N), phosphoprotein (P) and receptor binding haemagglutinin (H) gene of the ZJ7 isolate clearly showed it is joins to the Asia 1 group cluster of CDV strains, the predominant genotype in China. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Chile | 1 | 2% |
United States | 1 | 2% |
Unknown | 43 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 24% |
Student > Master | 7 | 16% |
Student > Ph. D. Student | 5 | 11% |
Student > Bachelor | 4 | 9% |
Professor > Associate Professor | 3 | 7% |
Other | 5 | 11% |
Unknown | 10 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 29% |
Veterinary Science and Veterinary Medicine | 7 | 16% |
Medicine and Dentistry | 6 | 13% |
Environmental Science | 2 | 4% |
Immunology and Microbiology | 2 | 4% |
Other | 3 | 7% |
Unknown | 12 | 27% |