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In vitro effects of 3 % hypertonic saline and 20 % mannitol on canine whole blood coagulation and platelet function

Overview of attention for article published in BMC Veterinary Research, September 2015
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Title
In vitro effects of 3 % hypertonic saline and 20 % mannitol on canine whole blood coagulation and platelet function
Published in
BMC Veterinary Research, September 2015
DOI 10.1186/s12917-015-0555-x
Pubmed ID
Authors

Katja-Nicole Adamik, Emmanuelle Butty, Judith Howard

Abstract

Hyperosmolar therapy, using either mannitol or hypertonic saline (HTS), is considered the treatment of choice for intracranial hypertension. However, hyperosmolar agents may impair coagulation and platelet function, limiting their use in patients at risk for hemorrhage. Despite this, studies evaluating the effects of mannitol compared to other hyperosmolar agents in dogs are largely lacking. The aim of this study was to compare the in vitro effects on global hemostasis and platelet function of 20 % mannitol and 3 % HTS on canine blood. Citrated whole blood from 15 healthy dogs was diluted with 0.9 % saline, 20 % mannitol and 3 % HTS in ratios of 1:16 and 1:8. Rotational thromboelastometry (ROTEM) was used to assess clotting time (CT), clot formation time (CFT) and maximal clot firmness (MCF) following extrinsic activation (Ex-tem) and after platelet inhibition (Fib-tem). A platelet function analyzer (PFA-100) was used to assess closure time (CtPFA). No significant differences were observed between untreated whole blood and samples diluted with saline. Samples diluted with both mannitol and HTS were hypocoagulable compared to untreated whole blood samples. At a dilution of 1:16, no significant differences were found between any measured parameter in samples diluted with saline compared to mannitol or HTS. At a 1:8 dilution, CtPFA was prolonged in samples diluted with mannitol and HTS compared to saline, and CtPFA was prolonged more with mannitol than HTS. Ex-tem CT was increased at a 1:8 dilution with mannitol compared to HTS. Ex-tem CFT was prolonged at a 1:8 dilution with both agents compared to saline, and was prolonged more with mannitol than HTS. Ex-tem MCF was reduced at a 1:8 dilution with both agents compared to saline. Data in this study indicate that both mannitol and HTS affect canine platelet function and whole blood coagulation in vitro in a dose-dependent fashion. The most pronounced effects were observed after high dilutions with mannitol, which impaired platelet aggregation, clot formation time, clot strength, and fibrin formation significantly more than HTS. Further in vivo studies are necessary before recommendations can be made.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Austria 1 4%
Unknown 25 96%

Demographic breakdown

Readers by professional status Count As %
Other 3 12%
Student > Doctoral Student 3 12%
Student > Master 3 12%
Student > Ph. D. Student 3 12%
Student > Postgraduate 3 12%
Other 2 8%
Unknown 9 35%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 9 35%
Biochemistry, Genetics and Molecular Biology 2 8%
Medicine and Dentistry 2 8%
Psychology 1 4%
Nursing and Health Professions 1 4%
Other 2 8%
Unknown 9 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2015.
All research outputs
#20,293,238
of 22,829,683 outputs
Outputs from BMC Veterinary Research
#2,418
of 3,050 outputs
Outputs of similar age
#230,517
of 274,661 outputs
Outputs of similar age from BMC Veterinary Research
#44
of 53 outputs
Altmetric has tracked 22,829,683 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,050 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 53 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.