Title |
The role of miR-29c/B7-H3 axis in children with allergic asthma
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Published in |
Journal of Translational Medicine, August 2018
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DOI | 10.1186/s12967-018-1590-8 |
Pubmed ID | |
Authors |
Xinxing Zhang, Xin Zhao, Huiming Sun, Yongdong Yan, Li Huang, Wenjin Gu, Wujun Jiang, Yuqing Wang, Canhong Zhu, Wei Ji, Chuangli Hao, Zhengrong Chen |
Abstract |
MicroRNAs play roles in the pathogenesis of bronchial asthma. However, the mechanism of miR-29c in allergic asthma remains unclear. This study is to elucidate the regulation of Th cell differentiation by miR-29c in mononuclear macrophages. A total of 52 children with asthma exacerbation and 26 children as controls were enrolled in the study. CD14+ monocytes were isolated from the peripheral blood. Differential expressions of microRNAs were evaluated using microarray analysis and miR-29c expression in monocytes was determined by qRT-PCR. The plasma B7-H3 was determined by ELISA. Transfection studies and luciferase reporter assay were performed to confirm target gene of miR-29c and its function. Compared to controls, 88 miRNAs in blood monocytes were up-regulated and 41 miRNAs down-regulated including miR-29c in asthma children. Children with asthma exacerbation had significantly lower level of miR-29c and higher level of plasma B7-H3 compared to controls (both P < 0.05). Functional studies based on luciferase reporter assay and immunofluorescence staining suggest that B7-H3 is the direct target of miR-29c and transfection anti-miR-29c into macrophages could enhance ROR-γt and GATA-3 expression in co-cultured CD4+ T cells and increase levels of IL-4 and IL-17 in supernatants. The axis of miR-29c/B7-H3 plays an important role in children with asthma through regulating Th2/Th17 cell differentiation and may provide new targets for treatment of asthma. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 3 | 19% |
Student > Master | 3 | 19% |
Researcher | 3 | 19% |
Student > Ph. D. Student | 1 | 6% |
Student > Bachelor | 1 | 6% |
Other | 1 | 6% |
Unknown | 4 | 25% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 4 | 25% |
Agricultural and Biological Sciences | 3 | 19% |
Medicine and Dentistry | 3 | 19% |
Biochemistry, Genetics and Molecular Biology | 1 | 6% |
Materials Science | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 25% |