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Identification of ISMyo2, a novel insertion sequence element of IS21 family and its diagnostic potential for detection of Mycobacterium yongonense

Overview of attention for article published in BMC Genomics, October 2015
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Mentioned by

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3 tweeters
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1 Facebook page

Citations

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3 Dimensions

Readers on

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8 Mendeley
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Title
Identification of ISMyo2, a novel insertion sequence element of IS21 family and its diagnostic potential for detection of Mycobacterium yongonense
Published in
BMC Genomics, October 2015
DOI 10.1186/s12864-015-1978-2
Pubmed ID
Authors

Byoung-Jun Kim, Kijeong Kim, Bo-Ram Kim, Yoon-Hoh Kook, Bum-Joon Kim

Abstract

Mycobacterium yongonense, as a novel member of the M. avium complex (MAC), was recently reported to be isolated from human specimens in South Korea and Italy. Due to its close relatedness to other MAC members, particularly M. intracellulare in taxonomic aspects, the development of a novel diagnostic method for its specific detection is necessary for clinical or epidemiologic purposes. Using the Mycobacterium yongonense genome information, we have identified a novel IS-element, ISMyo2. Targeting the ISMyo2 sequence, we developed a real-time PCR method and applied the technique to Mycobacterial genomic DNA. To identify proper nucleic acid targets for the diagnosis, comparisons of all insertion sequence (IS) elements of 3 M. intracellulare and 3 M. yongonense strains, whose complete genome sequences we reported recently, led to the selection of a novel target gene, the M. yongonense-specific IS element, ISMyo2 (2,387 bp), belonging to the IS21 family. Next, we developed a real-time PCR method using SYBR green I for M. yongonense-specific detection targeting ISMyo2, producing a 338-bp amplicon. When this assay was applied to 28 Mycobacterium reference strains and 63 MAC clinical isolates, it produced amplicons in only the 6 M. yongonense strains, showing a sensitivity of 100 fg of genomic DNA, suggesting its feasibility as a diagnostic method for M. yongonense strains. We identified a novel ISMyo2 IS element belonging to the IS21 family specific to M. yongonense strains via genome analysis, and a real-time PCR method based on its sequences was developed.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 25%
Other 1 13%
Librarian 1 13%
Student > Doctoral Student 1 13%
Student > Ph. D. Student 1 13%
Other 1 13%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 25%
Computer Science 1 13%
Biochemistry, Genetics and Molecular Biology 1 13%
Veterinary Science and Veterinary Medicine 1 13%
Immunology and Microbiology 1 13%
Other 0 0%
Unknown 2 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2016.
All research outputs
#4,187,897
of 7,976,486 outputs
Outputs from BMC Genomics
#3,663
of 5,712 outputs
Outputs of similar age
#126,281
of 242,399 outputs
Outputs of similar age from BMC Genomics
#287
of 372 outputs
Altmetric has tracked 7,976,486 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,712 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 242,399 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 372 others from the same source and published within six weeks on either side of this one. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.