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The notch pathway promotes NF-κB activation through Asb2 in T cell acute lymphoblastic leukemia cells

Overview of attention for article published in Cellular & Molecular Biology Letters, August 2018
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Title
The notch pathway promotes NF-κB activation through Asb2 in T cell acute lymphoblastic leukemia cells
Published in
Cellular & Molecular Biology Letters, August 2018
DOI 10.1186/s11658-018-0102-4
Pubmed ID
Authors

Wei Wu, Li Nie, Li Zhang, Yan Li

Abstract

Oncogenic Notch1 is known to activate the NF-κB pathway in T cell acute lymphoblastic leukemia (T-ALL) and to up-regulate the transcription of Asb2α, a specificity factor for an E3 ubiquitin ligase complex that plays an important role in hematopoietic differentiation. Therefore, we hypothesize that Notch1 might regulate the NF-κB pathway through Asb2α. The study involved down-regulation of Notch1 in T-ALL cell lines (CCRF-CEM cells and MOLT-4 cells) through treatment with gamma-secretase inhibitor (GSI) as well as the modulation of Asb2 in CCRF-CEM cells and MOLT-4 cells through transduction with lentivirus carrying Asb2 or Asb2-shRNA. Experiments using real-time PCR, western blot and co-immunoprecipitation were performed to evaluate the expression levels of related genes. Cell proliferation and apoptosis were measured while the expression of Asb2 was enhanced or inhibited. Here, we demonstrated for the first time that Notch1 can activate the transcription of Asb2α, which then stimulates activation of NF-κB in T-ALL cells. Asb2α exerts its effects by inducing degradation and dissociation of IκBα from NF-κB in T-ALL cells. Moreover, specific suppression of Asb2α expression can promote apoptosis and inhibit proliferation of T-ALL cells. Notch1 modulates the NF-κB pathway through Asb2α, indicating that Asb2α inhibition is a promising option for targeted therapy against T-ALL.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 40%
Student > Bachelor 1 10%
Lecturer 1 10%
Professor 1 10%
Student > Postgraduate 1 10%
Other 0 0%
Unknown 2 20%
Readers by discipline Count As %
Immunology and Microbiology 4 40%
Biochemistry, Genetics and Molecular Biology 2 20%
Agricultural and Biological Sciences 1 10%
Medicine and Dentistry 1 10%
Unknown 2 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 August 2018.
All research outputs
#10,624,139
of 13,347,801 outputs
Outputs from Cellular & Molecular Biology Letters
#96
of 220 outputs
Outputs of similar age
#200,119
of 267,902 outputs
Outputs of similar age from Cellular & Molecular Biology Letters
#1
of 1 outputs
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So far Altmetric has tracked 220 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
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