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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Characterizing the pathotype of neonatal meningitis causing Escherichia coli (NMEC)
|
|---|---|
| Published in |
BMC Microbiology, October 2015
|
| DOI | 10.1186/s12866-015-0547-9 |
| Pubmed ID | |
| Authors |
D. S. S. Wijetunge, S. Gongati, C. DebRoy, K. S. Kim, P. O. Couraud, I. A. Romero, B. Weksler, S. Kariyawasam |
| Abstract |
Neonatal meningitis-causing Escherichia coli (NMEC) is the predominant Gram-negative bacterial pathogen associated with meningitis in newborn infants. High levels of heterogeneity and diversity have been observed in the repertoire of virulence traits and other characteristics among strains of NMEC making it difficult to define the NMEC pathotype. The objective of the present study was to identify genotypic and phenotypic characteristics of NMEC that can be used to distinguish them from commensal E. coli. A total of 53 isolates of NMEC obtained from neonates with meningitis and 48 isolates of fecal E. coli obtained from healthy individuals (HFEC) were comparatively evaluated using five phenotypic (serotyping, serum bactericidal assay, biofilm assay, antimicorbial susceptibility testing, and in vitro cell invasion assay) and three genotypic (phylogrouping, virulence genotyping, and pulsed-field gel electrophoresis) methods. A majority (67.92 %) of NMEC belonged to B2 phylogenetic group whereas 59 % of HFEC belonged to groups A and D. Serotyping revealed that the most common O and H types present in NMEC tested were O1 (15 %), O8 (11.3 %), O18 (13.2 %), and H7 (25.3 %). In contrast, none of the HFEC tested belonged to O1 or O18 serogroups. The most common serogroup identified in HFEC was O8 (6.25 %). The virulence genotyping reflected that more than 70 % of NMEC carried kpsII, K1, neuC, iucC, sitA, and vat genes with only less than 27 % of HFEC possessing these genes. All NMEC and 79 % of HFEC tested were able to invade human cerebral microvascular endothelial cells. No statistically significant difference was observed in the serum resistance phenotype between NMEC and HFEC. The NMEC strains demonstrated a greater ability to form biofilms in Luria Bertani broth medium than did HFEC (79.2 % vs 39.9 %). The results of our study demonstrated that virulence genotyping and phylogrouping may assist in defining the potential NMEC pathotype. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 140 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 140 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 12% |
| Student > Master | 16 | 11% |
| Student > Bachelor | 15 | 11% |
| Researcher | 14 | 10% |
| Student > Doctoral Student | 8 | 6% |
| Other | 20 | 14% |
| Unknown | 50 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 23 | 16% |
| Biochemistry, Genetics and Molecular Biology | 18 | 13% |
| Agricultural and Biological Sciences | 16 | 11% |
| Medicine and Dentistry | 11 | 8% |
| Veterinary Science and Veterinary Medicine | 7 | 5% |
| Other | 9 | 6% |
| Unknown | 56 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2015.
All research outputs
#20,294,248
of 22,830,751 outputs
Outputs from BMC Microbiology
#2,689
of 3,191 outputs
Outputs of similar age
#234,283
of 279,406 outputs
Outputs of similar age from BMC Microbiology
#57
of 75 outputs
Altmetric has tracked 22,830,751 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,191 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 75 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.