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CNS-border associated macrophages respond to acute ischemic stroke attracting granulocytes and promoting vascular leakage

Overview of attention for article published in Acta Neuropathologica Communications, August 2018
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Title
CNS-border associated macrophages respond to acute ischemic stroke attracting granulocytes and promoting vascular leakage
Published in
Acta Neuropathologica Communications, August 2018
DOI 10.1186/s40478-018-0581-6
Pubmed ID
Authors

Jordi Pedragosa, Angélica Salas-Perdomo, Mattia Gallizioli, Roger Cugota, Francesc Miró-Mur, Ferran Briansó, Carles Justicia, Fernando Pérez-Asensio, Leonardo Marquez-Kisinousky, Xabier Urra, Anna Gieryng, Bozena Kaminska, Angel Chamorro, Anna M. Planas

Abstract

The central nervous system (CNS) contains several types of immune cells located in specific anatomic compartments. Macrophages reside at the CNS borders surrounding the brain vessels, in leptomeningeal spaces and the choroid plexus, where they interact with the vasculature and play immunological surveillance and scavenging functions. We investigated the phenotypic changes and role of these macrophages in response to acute ischemic stroke. Given that CD163 expression is a hallmark of perivascular and meningeal macrophages in the rat and human brain, we isolated CD163+ brain macrophages by fluorescence activated cell sorting. We obtained CD163+ cells from control rats and 16 h following transient middle cerebral artery occlusion, after verifying that infiltration of CD163+ peripheral myeloid cells is negligible at this acute time point. Transcriptome analysis of the sorted CD163+ cells identified ischemia-induced upregulation of the hypoxia inducible factor-1 pathway and induction of genes encoding for extracellular matrix components and leukocyte chemoattractants, amongst others. Using a cell depletion strategy, we found that CNS border-associated macrophages participate in granulocyte recruitment, promote the expression of vascular endothelial growth factor (VEGF), increase the permeability of pial and cortical blood vessels, and contribute to neurological dysfunction in the acute phase of ischemia/reperfusion. We detected VEGF expression surrounding blood vessels and in some CD163+ perivascular macrophages in the brain tissue of ischemic stroke patients deceased one day after stroke onset. These findings show ischemia-induced reprogramming of the gene expression profile of CD163+ macrophages that has a rapid impact on leukocyte chemotaxis and blood-brain barrier integrity, and promotes neurological impairment in the acute phase of stroke.

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The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 125 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 125 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 23 18%
Student > Ph. D. Student 20 16%
Student > Master 12 10%
Student > Bachelor 11 9%
Student > Doctoral Student 9 7%
Other 19 15%
Unknown 31 25%
Readers by discipline Count As %
Neuroscience 31 25%
Immunology and Microbiology 13 10%
Biochemistry, Genetics and Molecular Biology 11 9%
Agricultural and Biological Sciences 10 8%
Medicine and Dentistry 9 7%
Other 11 9%
Unknown 40 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2018.
All research outputs
#13,624,398
of 23,099,576 outputs
Outputs from Acta Neuropathologica Communications
#1,031
of 1,398 outputs
Outputs of similar age
#169,774
of 331,391 outputs
Outputs of similar age from Acta Neuropathologica Communications
#30
of 43 outputs
Altmetric has tracked 23,099,576 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,398 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 12.8. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,391 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.