↓ Skip to main content

Weevil endosymbiont dynamics is associated with a clamping of immunity

Overview of attention for article published in BMC Genomics, October 2015
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (75th percentile)
  • High Attention Score compared to outputs of the same age and source (81st percentile)

Mentioned by

twitter
10 X users

Citations

dimensions_citation
33 Dimensions

Readers on

mendeley
62 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Weevil endosymbiont dynamics is associated with a clamping of immunity
Published in
BMC Genomics, October 2015
DOI 10.1186/s12864-015-2048-5
Pubmed ID
Authors

Florent Masson, Yves Moné, Aurélien Vigneron, Agnès Vallier, Nicolas Parisot, Carole Vincent-Monégat, Séverine Balmand, Marie-Christine Carpentier, Anna Zaidman-Rémy, Abdelaziz Heddi

Abstract

Insects subsisting on nutritionally unbalanced diets have evolved long-term mutualistic relationships with intracellular symbiotic bacteria (endosymbionts). The endosymbiont population load undergoes changes along with insect development. In the cereal weevil Sitophilus oryzae, the midgut endosymbionts Sodalis pierantonius drastically multiply following adult metamorphosis and rapidly decline until total elimination when the insect achieves its cuticle synthesis. Whilst symbiont load was shown to timely meet insect metabolic needs, little is known about the host molecular and immune processes underlying this dynamics. We performed RNA sequencing analysis on weevil midguts at three representative phases of the endosymbiont dynamics (i.e. increase, climax and decrease). To screen genes which transcriptional changes are specifically related to symbiont dynamics and not to the intrinsic development of the midgut, we further have monitored by RT-qPCR sixteen gene transcript levels in symbiotic and artificially non-symbiotic (aposymbiotic) weevils. We also localized the endosymbionts during the elimination process by fluorescence microscopy. Functional analysis of the host differentially expressed genes by RNA sequencing showed that the main transcriptional changes occur during endosymbiont growth phase and affect cell proliferation, apoptosis, autophagy, phagocytosis, and metabolism of fatty acids and nucleic acids. We also showed that symbiont dynamics alters the expression of several genes involved in insect development. Our results strengthened the implication of apoptosis and autophagy processes in symbiont elimination and recycling. Remarkably, apart from the coleoptericin A that is known to target endosymbionts and controls their division and location, no gene coding antimicrobial peptide was upregulated during the symbiont growth and elimination phases. We show that endosymbiont dynamics parallels numerous transcriptional changes in weevil developing adults and affects several biological processes, including metabolism and development. It also triggers cell apoptosis, autophagy and gut epithelial cell swelling and delamination. Strikingly, immunity is repressed during the whole process, presumably avoiding tissue inflammation and allowing insects to optimize nutrient recovery from recycled endosymbiont.

X Demographics

X Demographics

The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Czechia 1 2%
Slovenia 1 2%
Unknown 60 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 24%
Student > Ph. D. Student 12 19%
Student > Doctoral Student 11 18%
Student > Master 4 6%
Other 2 3%
Other 6 10%
Unknown 12 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 38 61%
Biochemistry, Genetics and Molecular Biology 6 10%
Immunology and Microbiology 3 5%
Nursing and Health Professions 1 2%
Unknown 14 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 October 2015.
All research outputs
#6,308,352
of 25,408,670 outputs
Outputs from BMC Genomics
#2,374
of 11,255 outputs
Outputs of similar age
#72,379
of 295,323 outputs
Outputs of similar age from BMC Genomics
#67
of 360 outputs
Altmetric has tracked 25,408,670 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 11,255 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 295,323 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 360 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.