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QsRNA-seq: a method for high-throughput profiling and quantifying small RNAs

Overview of attention for article published in Genome Biology, August 2018
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)

Mentioned by

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18 X users

Citations

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18 Dimensions

Readers on

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73 Mendeley
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1 CiteULike
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Title
QsRNA-seq: a method for high-throughput profiling and quantifying small RNAs
Published in
Genome Biology, August 2018
DOI 10.1186/s13059-018-1495-0
Pubmed ID
Authors

Alla Fishman, Dean Light, Ayelet T. Lamm

Abstract

The ability to profile and quantify small non-coding RNAs (sRNAs), specifically microRNAs (miRNAs), using high-throughput sequencing is challenging because of their small size. We developed QsRNA-seq, a method for preparation of sRNA libraries for high-throughput sequencing that overcomes this difficulty by enabling a gel-free separation of fragments shorter than 100 nt that differ only by 20 nt in length. The method allows the use of unique molecular identifiers for quantification and is more amenable to automation than gel-based methods. We show that QsRNA-seq gives very accurate, comprehensive, and reproducible results by looking at miRNAs in Caenorhabditis elegans embryos and larvae.

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X Demographics

The data shown below were collected from the profiles of 18 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 73 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 22%
Researcher 16 22%
Student > Bachelor 6 8%
Student > Master 6 8%
Student > Doctoral Student 4 5%
Other 4 5%
Unknown 21 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 22 30%
Agricultural and Biological Sciences 19 26%
Immunology and Microbiology 2 3%
Neuroscience 2 3%
Linguistics 1 1%
Other 4 5%
Unknown 23 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 October 2021.
All research outputs
#3,344,831
of 25,385,509 outputs
Outputs from Genome Biology
#2,395
of 4,468 outputs
Outputs of similar age
#63,795
of 341,562 outputs
Outputs of similar age from Genome Biology
#56
of 67 outputs
Altmetric has tracked 25,385,509 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,468 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 341,562 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 67 others from the same source and published within six weeks on either side of this one. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.