Title |
QsRNA-seq: a method for high-throughput profiling and quantifying small RNAs
|
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Published in |
Genome Biology, August 2018
|
DOI | 10.1186/s13059-018-1495-0 |
Pubmed ID | |
Authors |
Alla Fishman, Dean Light, Ayelet T. Lamm |
Abstract |
The ability to profile and quantify small non-coding RNAs (sRNAs), specifically microRNAs (miRNAs), using high-throughput sequencing is challenging because of their small size. We developed QsRNA-seq, a method for preparation of sRNA libraries for high-throughput sequencing that overcomes this difficulty by enabling a gel-free separation of fragments shorter than 100 nt that differ only by 20 nt in length. The method allows the use of unique molecular identifiers for quantification and is more amenable to automation than gel-based methods. We show that QsRNA-seq gives very accurate, comprehensive, and reproducible results by looking at miRNAs in Caenorhabditis elegans embryos and larvae. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 22% |
Israel | 2 | 11% |
France | 1 | 6% |
United Kingdom | 1 | 6% |
Unknown | 10 | 56% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 9 | 50% |
Scientists | 8 | 44% |
Science communicators (journalists, bloggers, editors) | 1 | 6% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 73 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 16 | 22% |
Researcher | 16 | 22% |
Student > Bachelor | 6 | 8% |
Student > Master | 6 | 8% |
Student > Doctoral Student | 4 | 5% |
Other | 4 | 5% |
Unknown | 21 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 22 | 30% |
Agricultural and Biological Sciences | 19 | 26% |
Immunology and Microbiology | 2 | 3% |
Neuroscience | 2 | 3% |
Linguistics | 1 | 1% |
Other | 4 | 5% |
Unknown | 23 | 32% |