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Preparation, characterization and immunological evaluation: canine parvovirus synthetic peptide loaded PLGA nanoparticles

Overview of attention for article published in Journal of Biomedical Science, October 2015
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2 tweeters

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44 Dimensions

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94 Mendeley
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Title
Preparation, characterization and immunological evaluation: canine parvovirus synthetic peptide loaded PLGA nanoparticles
Published in
Journal of Biomedical Science, October 2015
DOI 10.1186/s12929-015-0195-2
Pubmed ID
Authors

Serap Derman, Zeynep Akdeste Mustafaeva, Emrah Sefik Abamor, Melahat Bagirova, Adil Allahverdiyev

Abstract

Canine parvovirus 2 (CPV-2) remains a significant worldwide canine pathogen and the most common cause of viral enteritis in dogs. The 1 L15 and 7 L15 peptides overlap each other with QPDGGQPAV residues (7-15 of VP2 capsid protein of CPV) is shown to produce high immune response. PLGA nanoparticles were demonstrated to have special properties such as; controlled antigen release, protection from degradation, elimination of booster-dose and enhancing the cellular uptake by antigen presenting cells. Nevertheless, there is no study available in literature, about developing vaccine based on PLGA nanoparticles with adjuvant properties against CPV. Thus, the aim of the present study was to synthesize and characterize high immunogenic W-1 L19 peptide (from the VP2 capsid protein of CPV) loaded PLGA nanoparticle and to evaluate their in vitro immunogenic activity. PLGA nanoparticles were produced with 5.26 ± 0.05 % loading capacity and high encapsulation efficiency with 81.2 ± 3.1 %. Additionally, it was evaluated that free NPs and W-1 L19 peptide encapsulated PLGA nanoparticles have Z-ave of 183.9 ± 12.1 nm, 221.7 ± 15.8 nm and polydispersity index of 0.107 ± 0.08, 0.135 ± 0.12 respectively. It was determined that peptide loaded PLGA nanoparticles were successfully phagocytized by macrophage cells and increased NO production at 2-folds (*P < 0.05) in contrast to free peptide, and 3-folds (*P < 0.01) in contrast to control. In conclusion, for the first time, W-1 L19 peptide loaded PLGA nanoparticles were successfully synthesized and immunogenic properties evaluated. Obtained results showed that PLGA nanoparticles enhanced the capacity of W-1 L19 peptide to induce nitric oxide production in vitro due to its adjuvant properties. Depend on the obtained results, these nanoparticles can be accepted as potential vaccine candidate against Canine Parvovirus. Studies targeting PLGA nanoparticles based delivery system must be maintained in near future in order to develop new and more effective nano-vaccine formulations.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
South Africa 1 1%
Unknown 93 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 23 24%
Student > Master 14 15%
Student > Ph. D. Student 13 14%
Researcher 8 9%
Student > Doctoral Student 5 5%
Other 7 7%
Unknown 24 26%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 18 19%
Biochemistry, Genetics and Molecular Biology 15 16%
Agricultural and Biological Sciences 8 9%
Veterinary Science and Veterinary Medicine 7 7%
Chemistry 7 7%
Other 14 15%
Unknown 25 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2015.
All research outputs
#2,911,788
of 6,554,440 outputs
Outputs from Journal of Biomedical Science
#147
of 308 outputs
Outputs of similar age
#102,560
of 208,836 outputs
Outputs of similar age from Journal of Biomedical Science
#8
of 22 outputs
Altmetric has tracked 6,554,440 research outputs across all sources so far. This one has received more attention than most of these and is in the 52nd percentile.
So far Altmetric has tracked 308 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 208,836 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.