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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Loss of Transient Receptor Potential Melastatin 3 ion channel function in natural killer cells from Chronic Fatigue Syndrome/Myalgic Encephalomyelitis patients
|
|---|---|
| Published in |
Molecular Medicine, August 2018
|
| DOI | 10.1186/s10020-018-0046-1 |
| Pubmed ID | |
| Authors |
Hélène Cabanas, Katsuhiko Muraki, Natalie Eaton, Cassandra Balinas, Donald Staines, Sonya Marshall-Gradisnik |
| Abstract |
Chronic Fatigue Syndrome (CFS)/ Myalgic Encephalomyelitis (ME) is a debilitating disorder that is accompanied by reduced cytotoxic activity in natural killer (NK) cells. NK cells are an essential innate immune cell, responsible for recognising and inducing apoptosis of tumour and virus infected cells. Calcium is an essential component in mediating this cellular function. Transient Receptor Potential Melastatin 3 (TRPM3) cation channels have an important regulatory role in mediating calcium influx to help maintain cellular homeostasis. Several single nucleotide polymorphisms have been reported in TRPM3 genes from isolated peripheral blood mononuclear cells, NK and B cells in patients with CFS/ME and have been proposed to correlate with illness presentation. Moreover, a significant reduction in both TRPM3 surface expression and intracellular calcium mobilisation in NK cells has been found in CFS/ME patients compared with healthy controls. Despite the functional importance of TRPM3, little is known about the ion channel function in NK cells and the epiphenomenon of CFS/ME. The objective of the present study was to characterise the TRPM3 ion channel function in NK cells from CFS/ME patients in comparison with healthy controls using whole cell patch-clamp techniques. NK cells were isolated from 12 age- and sex-matched healthy controls and CFS patients. Whole cell electrophysiology recording has been used to assess TRPM3 ion channel activity after modulation with pregnenolone sulfate and ononetin. We report a significant reduction in amplitude of TRPM3 current after pregnenolone sulfate stimulation in isolated NK cells from CFS/ME patients compared with healthy controls. In addition, we found pregnenolone sulfate-evoked ionic currents through TRPM3 channels were significantly modulated by ononetin in isolated NK cells from healthy controls compared with CFS/ME patients. TRPM3 activity is impaired in CFS/ME patients suggesting changes in intracellular Ca2+ concentration, which may impact NK cellular functions. This investigation further helps to understand the intracellular-mediated roles in NK cells and confirm the potential role of TRPM3 ion channels in the aetiology and pathomechanism of CFS/ME. |
X Demographics
The data shown below were collected from the profiles of 58 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 11 | 19% |
| United Kingdom | 7 | 12% |
| Netherlands | 2 | 3% |
| Canada | 2 | 3% |
| Australia | 1 | 2% |
| Norway | 1 | 2% |
| Mexico | 1 | 2% |
| Germany | 1 | 2% |
| Sweden | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 29 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 48 | 83% |
| Practitioners (doctors, other healthcare professionals) | 6 | 10% |
| Scientists | 3 | 5% |
| Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 13% |
| Student > Doctoral Student | 4 | 10% |
| Other | 4 | 10% |
| Student > Master | 4 | 10% |
| Student > Bachelor | 3 | 8% |
| Other | 6 | 15% |
| Unknown | 13 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 11 | 28% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Chemical Engineering | 2 | 5% |
| Immunology and Microbiology | 2 | 5% |
| Nursing and Health Professions | 1 | 3% |
| Other | 7 | 18% |
| Unknown | 14 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 38. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2023.
All research outputs
#1,091,599
of 25,641,627 outputs
Outputs from Molecular Medicine
#33
of 1,208 outputs
Outputs of similar age
#22,695
of 342,263 outputs
Outputs of similar age from Molecular Medicine
#1
of 9 outputs
Altmetric has tracked 25,641,627 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,208 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 342,263 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them