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Molecular study on the carAB operon reveals that carB gene is required for swimming and biofilm formation in Xanthomonas citri subsp. citri

Overview of attention for article published in BMC Microbiology, October 2015
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  • High Attention Score compared to outputs of the same age and source (81st percentile)

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Title
Molecular study on the carAB operon reveals that carB gene is required for swimming and biofilm formation in Xanthomonas citri subsp. citri
Published in
BMC Microbiology, October 2015
DOI 10.1186/s12866-015-0555-9
Pubmed ID
Authors

Tao Zhuo, Wei Rou, Xue Song, Jing Guo, Xiaojing Fan, Gicharu Gibson Kamau, Huasong Zou

Abstract

The carA and carB genes code the small and large subunits of carbamoyl-phosphate synthase (CPS) that responsible for arginine and pyrimidine production. The purpose of this work was to study the gene organization and expression pattern of carAB operon, and the biological functions of carA and carB genes in Xanthomonas citri subsp. citri. RT-PCR method was employed to identify the full length of carAB operon transcript in X. citri subsp. citri. The promoter of carAB operon was predicted and analyzed its activity by fusing a GUS reporter gene. The swimming motility was tested on 0.25 % agar NY plates with 1 % glucose. Biofilm was measured by cell adhesion to polyvinyl chloride 96-well plate. The results indicated that carAB operon was composed of five gene members carA-orf-carB-greA-rpfE. A single promoter was predicted from the nucleotide sequence upstream of carAB operon, and its sensitivity to glutamic acid, uracil and arginine was confirmed by fusing a GUS reporter gene. Deletion mutagenesis of carB gene resulted in reduced abilities in swimming on soft solid media and in forming biofilm on polystyrene microtiter plates. From these results, we concluded that carAB operon was involved in multiple biological processes in X. citri subsp. citri.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 4%
France 1 4%
Unknown 24 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 27%
Student > Bachelor 5 19%
Student > Ph. D. Student 2 8%
Professor 1 4%
Other 1 4%
Other 2 8%
Unknown 8 31%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 27%
Biochemistry, Genetics and Molecular Biology 5 19%
Environmental Science 1 4%
Mathematics 1 4%
Sports and Recreations 1 4%
Other 0 0%
Unknown 11 42%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2023.
All research outputs
#7,990,681
of 24,727,020 outputs
Outputs from BMC Microbiology
#887
of 3,405 outputs
Outputs of similar age
#94,991
of 289,539 outputs
Outputs of similar age from BMC Microbiology
#15
of 75 outputs
Altmetric has tracked 24,727,020 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 3,405 research outputs from this source. They receive a mean Attention Score of 4.2. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 289,539 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 75 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.