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Multilocus sequence typing of Streptococcus thermophilus from naturally fermented dairy foods in China and Mongolia

Overview of attention for article published in BMC Microbiology, October 2015
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  • High Attention Score compared to outputs of the same age and source (85th percentile)

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1 patent

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Title
Multilocus sequence typing of Streptococcus thermophilus from naturally fermented dairy foods in China and Mongolia
Published in
BMC Microbiology, October 2015
DOI 10.1186/s12866-015-0551-0
Pubmed ID
Authors

Jie Yu, Zhihong Sun, Wenjun Liu, Xiaoxia Xi, Yuqin Song, Haiyan Xu, Qiang Lv, Qiuhua Bao, Bilige Menghe, Tiansong Sun

Abstract

Streptococcus thermophilus is a major dairy starter used for manufacturing of dairy products. In the present study, we developed a multilocus sequence typing (MLST) scheme for this important food bacterium. Sequences of 10 housekeeping genes (carB, clpX, dnaA, murC, murE, pepN, pepX, pyrG, recA, and rpoB) were obtained for 239 S. thermophilus strains, which were isolated from home-made fermented dairy foods in 18 different regions of Mongolia and China. All 10 genes of S. thermophilus were sequenced, aligned, and defined sequence types (STs) using the BioNumerics Software. The nucleotide diversity was calculated by START v2.0. The population structure, phylogenetic relationships and the role of recombination were inferred using ClonalFrame v1.2, SplitsTree 4.0 and Structure v2.3. The 239 S. thermophilus isolates and 18 reference strains could be assigned into 119 different STs, which could be further separated into 16 clonal complexes (CCs) and 38 singletons. Among the 10 loci, a total of 132 polymorphic sites were detected. The standardized index of association (I A (S)  = 0.0916), split-decomposition and ρ/θ (relative frequency of occurrence of recombination and mutation) and r/m value (relative impact of recombination and mutation in the diversification) confirms that recombination may have occurred, but it occurred at a low frequency in these 10 loci. Phylogenetic trees indicated that there were five lineages in the S. thermophilus isolates used in our study. MSTree and ClonalFrame tree analyses suggest that the evolution of S. thermophilus isolates have little relationship with geographic locality, but revealed no association with the types of fermented dairy product. Phylogenetic analysis of 36 whole genome strains (18 S. thermophilus, 2 S. vestibularis and 16 S. salivarius strains) indicated that our MLST scheme could clearly separate three closely related species within the salivarius group and is suitable for analyzing the population structure of the other two species in the salivarius group. Our newly developed MLST scheme improved the understanding on the genetic diversity and population structure of the S. thermophilus, as well as provided useful information for further studies on the genotyping and evolutionary research for S. thermophilus strains with global diversity.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 4%
Unknown 26 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 22%
Professor 3 11%
Student > Master 3 11%
Researcher 3 11%
Student > Ph. D. Student 2 7%
Other 4 15%
Unknown 6 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 22%
Agricultural and Biological Sciences 5 19%
Chemical Engineering 1 4%
Unspecified 1 4%
Nursing and Health Professions 1 4%
Other 3 11%
Unknown 10 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 May 2020.
All research outputs
#6,426,255
of 22,831,537 outputs
Outputs from BMC Microbiology
#717
of 3,191 outputs
Outputs of similar age
#81,348
of 284,375 outputs
Outputs of similar age from BMC Microbiology
#11
of 77 outputs
Altmetric has tracked 22,831,537 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 3,191 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 284,375 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.