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Diethylnitrosamine induces lung adenocarcinoma in FVB/N mouse

Overview of attention for article published in BMC Cancer, February 2018
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Title
Diethylnitrosamine induces lung adenocarcinoma in FVB/N mouse
Published in
BMC Cancer, February 2018
DOI 10.1186/s12885-018-4068-4
Pubmed ID
Authors

Zsolt Mervai, Krisztina Egedi, Ilona Kovalszky, Kornélia Baghy

Abstract

Diethylnitrosamine is a well known carcinogen that induces cancers of various organs in mice and rats. Using FVB/N mouse strain, here we show that diethylnitrosamine induces primarily lung adenocarcinomas with modest tumor development in the liver, offering a new model to study chemical carcinogenesis in the lung. Animals were exposed to a single high dose of diethylnitrosamine, and more than 70% of the mice developed lung cancer. To obtain a new transplantable tumor line, pieces of primary tumors were inoculated and maintained subcutaneously in the same mouse strain. We used immunohistochemistry to characterize the tumor for main lung adenocarcinoma markers. We searched for mutations in KRAS exon 2 and EGFR exon 19, 21 with Sanger sequencing. We also compared the normal lung tissue with the diethylnitrosamine induced primary adenocarcinoma, and with the subcutaneously maintained adenocarcinoma using Western blot technique for main cell cycle markers and to identify the main pathways. Primary and subcutaneous tumors express cytokeratin-7 and thyroid transcription factor-1, markers characteristic to lung adenocarcinoma. In addition, no mutations were found in the hot spot regions of KRAS and EGFR genes. We found high mTOR activation, but the level of p-Akt Ser473 and p-Akt Thr308 decreased in the tumorous samples. We established a new lung adenocarcinoma model using FVB/N mouse strain and diethylnitrosamine. We believe that this new model system would be highly useful in lung cancer research.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 25%
Student > Master 1 8%
Student > Ph. D. Student 1 8%
Other 1 8%
Student > Postgraduate 1 8%
Other 0 0%
Unknown 5 42%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 17%
Medicine and Dentistry 2 17%
Environmental Science 1 8%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Chemistry 1 8%
Other 0 0%
Unknown 5 42%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2018.
All research outputs
#10,664,945
of 13,406,897 outputs
Outputs from BMC Cancer
#3,325
of 5,040 outputs
Outputs of similar age
#200,355
of 268,042 outputs
Outputs of similar age from BMC Cancer
#1
of 1 outputs
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