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Epidermal growth factor receptor status and Notch inhibition in non-small cell lung cancer cells

Overview of attention for article published in Journal of Biomedical Science, October 2015
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Title
Epidermal growth factor receptor status and Notch inhibition in non-small cell lung cancer cells
Published in
Journal of Biomedical Science, October 2015
DOI 10.1186/s12929-015-0196-1
Pubmed ID
Authors

Efstathia Giannopoulou, Achilleas Nikolakopoulos, Dimitra Kotsirilou, Angeliki Lampropoulou, Sofia Raftopoulou, Evangelia Papadimitriou, Achilleas D. Theocharis, Thomas Makatsoris, Konstantinos Fasseas, Haralabos P. Kalofonos

Abstract

Notch may behave as an oncogene or a tumor suppressor gene in lung cancer cells. Notch receptor undergoes cleavage by enzymes, including γ-secretase, generating the active Notch intracellular domain (NICD). The aim of the present study was to investigate the effect of DAPT, a γ-secretase inhibitor, in non-small cell lung cancer (NSCLC) cells, as well as the impact of epidermal growth factor (EGF) that is over-expressed by NSCLC cells, on Notch signaling. H23, A549, H661 and HCC827 human NSCLC cell lines were used, expressing various NICD and EGF receptor (EGFR) protein levels. DAPT decreased the number of H661 cells in a concentration-dependent manner, while it had a small effect on H23 and A549 cells and no effect on HCC827 cells that carry mutated EGFR. Notch inhibition did not affect the stimulatory effect of EGF on cell proliferation, while EGF prevented DAPT-induced NICD decrease in H23 and H661 cells. The type of cell death induced by DAPT seems to depend on the cell type. Our data indicate that inhibition of Notch cleavage may not affect cell number in the presence of EGFR mutations and that EGFR may affect Notch signalling suggesting that a dual inhibition of these pathways might be promising in NSCLC.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 31%
Student > Doctoral Student 2 15%
Researcher 2 15%
Student > Master 1 8%
Professor 1 8%
Other 0 0%
Unknown 3 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 46%
Biochemistry, Genetics and Molecular Biology 2 15%
Economics, Econometrics and Finance 1 8%
Medicine and Dentistry 1 8%
Engineering 1 8%
Other 0 0%
Unknown 2 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 October 2015.
All research outputs
#22,759,452
of 25,373,627 outputs
Outputs from Journal of Biomedical Science
#969
of 1,101 outputs
Outputs of similar age
#252,368
of 294,728 outputs
Outputs of similar age from Journal of Biomedical Science
#22
of 25 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,101 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 294,728 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.