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A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates

Overview of attention for article published in BMC Microbiology, October 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (70th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

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5 tweeters

Citations

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19 Dimensions

Readers on

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28 Mendeley
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Title
A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates
Published in
BMC Microbiology, October 2015
DOI 10.1186/s12866-015-0576-4
Pubmed ID
Authors

Roghaieh Ashrafi, Katja Pulkkinen, Lotta-Riina Sundberg, Nina Pekkala, Tarmo Ketola

Abstract

Columnaris disease caused by Flavobacterium columnare is a serious problem in aquaculture, annually causing large economic losses around the world. Despite considerable research, the molecular epidemiology of F. columnare remains poorly understood. We investigated the population structure and spatiotemporal changes in the genetic diversity of F. columnare population in Finland by using a multilocus sequence typing (MLST) and analysis (MLSA) based on DNA sequence variation within six housekeeping genes. A total of 83 strains of F. columnare were collected from eight different areas located across the country between 2003 and 2012. Partial sequencing of six housekeeping genes (trpB, tuf, atpA, rpoD, gyrB and dnaK) revealed eight sequence types and a moderate level of genetic diversity (H = 0.460). Phylogenetic analysis of the concatenated protein-encoding gene sequence data (ca. 3,509 nucleotides) formed two lineages, which could be further divided into five clusters. All analysed F. columnare strains appeared to have a genetic origin distinct from that of another important fish pathogen form the genus Flavobacterium, F. psychrophilum. Although the value of the index of association between alleles, 0.292 (P < 0.001), supports some degree of clonality for this species in Finland, recombination has introduced molecular diversity to the population almost three times more than mutation. The results suggest that Finnish F. columnare strains have an epidemic population structure followed by clonal expansion of successful genotypes. Our study with reproducible methodology and comparable results establishes a robust framework for the discrimination and phylogenetic analysis of F. columnare isolates, which will help to improve our understanding about geographic distribution and epidemiology of columnaris disease.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 25%
Researcher 7 25%
Student > Master 5 18%
Professor > Associate Professor 2 7%
Student > Bachelor 2 7%
Other 3 11%
Unknown 2 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 36%
Biochemistry, Genetics and Molecular Biology 6 21%
Immunology and Microbiology 4 14%
Veterinary Science and Veterinary Medicine 1 4%
Unspecified 1 4%
Other 1 4%
Unknown 5 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2015.
All research outputs
#1,423,240
of 6,526,504 outputs
Outputs from BMC Microbiology
#226
of 1,194 outputs
Outputs of similar age
#60,896
of 207,527 outputs
Outputs of similar age from BMC Microbiology
#7
of 84 outputs
Altmetric has tracked 6,526,504 research outputs across all sources so far. Compared to these this one has done well and is in the 77th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,194 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 207,527 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 84 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.