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Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines

Overview of attention for article published in Journal of Venomous Animals and Toxins including Tropical Diseases, November 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#33 of 311)
  • Good Attention Score compared to outputs of the same age (76th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

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3 tweeters
facebook
7 Facebook pages
googleplus
1 Google+ user

Citations

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25 Dimensions

Readers on

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60 Mendeley
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Title
Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines
Published in
Journal of Venomous Animals and Toxins including Tropical Diseases, November 2015
DOI 10.1186/s40409-015-0044-5
Pubmed ID
Authors

Cássio Prinholato da Silva, Tássia R. Costa, Raquel M. Alves Paiva, Adélia C. O. Cintra, Danilo L. Menaldo, Lusânia M. Greggi Antunes, Suely V. Sampaio

Abstract

Phospholipases A2 (PLA2s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA2 isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines. The cytotoxic effects of BthTX-I were evaluated on the tumor cell lines HL-60 (promyelocytic leukemia), HepG2 (human hepatocellular carcinoma), PC-12 (murine pheochromocytoma) and B16F10 (murine melanoma) using the MTT method. Flow cytometry technique was used for the analysis of cell cycle alterations and death mechanisms (apoptosis and/or necrosis) induced in tumor cells after treatment with BthTX-I. It was observed that BthTX-I was cytotoxic to all evaluated tumor cell lines, reducing their viability in 40 to 50 %. The myotoxin showed modulating effects on the cell cycle of PC-12 and B16F10 cells, promoting delay in the G0/G1 phase. Additionally, flow cytometry analysis indicated cell death mainly by apoptosis. B16F10 was more susceptible to the effects of BthTX-I, with ~40 % of the cells analyzed in apoptosis, followed by HepG2 (~35 %), PC-12 (~25 %) and HL-60 (~4 %). These results suggest that BthTX-I presents antitumor properties that may be useful for developing new therapeutic strategies against cancer.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 60 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 21 35%
Student > Ph. D. Student 6 10%
Student > Master 6 10%
Researcher 5 8%
Student > Postgraduate 4 7%
Other 7 12%
Unknown 11 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 28%
Biochemistry, Genetics and Molecular Biology 17 28%
Medicine and Dentistry 5 8%
Chemistry 3 5%
Pharmacology, Toxicology and Pharmaceutical Science 3 5%
Other 5 8%
Unknown 10 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 November 2015.
All research outputs
#2,659,168
of 11,973,628 outputs
Outputs from Journal of Venomous Animals and Toxins including Tropical Diseases
#33
of 311 outputs
Outputs of similar age
#58,130
of 252,393 outputs
Outputs of similar age from Journal of Venomous Animals and Toxins including Tropical Diseases
#1
of 18 outputs
Altmetric has tracked 11,973,628 research outputs across all sources so far. Compared to these this one has done well and is in the 77th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 311 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,393 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.