↓ Skip to main content

Changes in alpha-amylase activity, concentration and isoforms in pigs after an experimental acute stress model: an exploratory study

Overview of attention for article published in BMC Veterinary Research, August 2018
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
2 tweeters

Citations

dimensions_citation
19 Dimensions

Readers on

mendeley
31 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Changes in alpha-amylase activity, concentration and isoforms in pigs after an experimental acute stress model: an exploratory study
Published in
BMC Veterinary Research, August 2018
DOI 10.1186/s12917-018-1581-2
Pubmed ID
Authors

María Dolores Contreras-Aguilar, Damián Escribano, Silvia Martínez-Subiela, Silvia Martínez-Miró, Jose Joaquín Cerón, Fernando Tecles

Abstract

Salivary alpha-amylase (sAA) is considered a non-invasive biomarker of acute stress that can be evaluated by changes in activity and concentration, and also by changes in its isoforms, although this last way of evaluation has never been used in veterinary medicine. This research evaluated the changes of sAA by three different ways in which sAA can be evaluated in an experimental acute stress model in six pigs based in a technique of temporarily restraining. These ways of evaluation were 1) activity by a spectrophotometric assay, 2) concentration by a fluorometric assay, and 3) isoforms of the enzyme by a Western blot. Although salivary cortisol significantly increased due to the stimulus of stress and all the pigs manifested signs of stress by high-pitched vocalization, sAA activity showed an increase of different degree in the six pigs after the stress stimulus, while sAA concentration showed decreases in four of the six pigs. sAA activity did not correlate with sAA concentration or salivary cortisol, and a low correlation was observed between sAA concentration and salivary cortisol (r = 0.48, p = 0.003). The inter-individual variability was higher in sAA activity than in sAA concentration and salivary cortisol. Finally, three possible isoforms of sAA at 154-160 kDa, 65-66 kDa and 59-60 kDa were observed that showed different dynamics after the stress induction. Although this pilot study's results should be taken with caution due to the low sample size, it reveals a different behavior between sAA activity and concentration in pig after an acute stressful stimulus leading to evident external signs of stress by high-pitched vocalization, and opens a new field for the evaluation of possible selected isoforms of sAA as potential biomarkers of stress.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 19%
Student > Postgraduate 4 13%
Researcher 3 10%
Other 2 6%
Student > Doctoral Student 2 6%
Other 7 23%
Unknown 7 23%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 7 23%
Agricultural and Biological Sciences 3 10%
Neuroscience 2 6%
Biochemistry, Genetics and Molecular Biology 2 6%
Medicine and Dentistry 2 6%
Other 3 10%
Unknown 12 39%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 August 2018.
All research outputs
#10,260,597
of 13,451,706 outputs
Outputs from BMC Veterinary Research
#1,080
of 1,975 outputs
Outputs of similar age
#184,028
of 266,291 outputs
Outputs of similar age from BMC Veterinary Research
#7
of 14 outputs
Altmetric has tracked 13,451,706 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,975 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,291 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.