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Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Overview of attention for article published in Virology Journal, November 2011
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  • Good Attention Score compared to outputs of the same age (76th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

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7 X users

Citations

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30 Dimensions

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77 Mendeley
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Title
Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice
Published in
Virology Journal, November 2011
DOI 10.1186/1743-422x-8-524
Pubmed ID
Authors

TN Athmaram, Shweta Saraswat, SR Santhosh, Anil Kumar Singh, VVS Suryanarayana, Raj Priya, N Gopalan, Manmohan Parida, PV Lakshmana Rao, R Vijayaraghavan

Abstract

Currently available vaccines for the pandemic Influenza A (H1N1) 2009 produced in chicken eggs have serious impediments viz limited availability, risk of allergic reactions and the possible selection of sub-populations differing from the naturally occurring virus, whereas the cell culture derived vaccines are time consuming and may not meet the demands of rapid global vaccination required to combat the present/future pandemic. Hemagglutinin (HA) based subunit vaccine for H1N1 requires the HA protein in glycosylated form, which is impossible with the commonly used bacterial expression platform. Additionally, bacterial derived protein requires extensive purification and refolding steps for vaccine applications. For these reasons an alternative heterologous system for rapid, easy and economical production of Hemagglutinin protein in its glycosylated form is required. The HA gene of novel H1N1 A/California/04/2009 was engineered for expression in Pichia pastoris as a soluble secreted protein. The full length HA- synthetic gene having α-secretory tag was integrated into P. pastoris genome through homologous recombination. The resultant Pichia clones having multiple copy integrants of the transgene expressed full length HA protein in the culture supernatant. The Recombinant yeast derived H1N1 HA protein elicited neutralising antibodies both in mice and rabbits. The sera from immunised animals also exhibited Hemagglutination Inhibition (HI) activity. Considering the safety, reliability and also economic potential of Pichia expression platform, our preliminary data indicates the feasibility of using this system as an alternative for large-scale production of recombinant influenza HA protein in the face of influenza pandemic threat.

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X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 1%
United States 1 1%
Italy 1 1%
Unknown 74 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 22%
Researcher 13 17%
Student > Bachelor 10 13%
Student > Master 9 12%
Student > Doctoral Student 4 5%
Other 9 12%
Unknown 15 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 23 30%
Biochemistry, Genetics and Molecular Biology 10 13%
Engineering 7 9%
Medicine and Dentistry 6 8%
Immunology and Microbiology 4 5%
Other 12 16%
Unknown 15 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 December 2011.
All research outputs
#6,243,883
of 22,659,164 outputs
Outputs from Virology Journal
#649
of 3,024 outputs
Outputs of similar age
#55,700
of 240,140 outputs
Outputs of similar age from Virology Journal
#20
of 100 outputs
Altmetric has tracked 22,659,164 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 3,024 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.5. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,140 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 100 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.