@RetoPaul Hollow helicoid DNA structures (40–700 nm) with SMLM, also shown with SIM and multiple DNA dyes in the same paper https://t.co/J6BLACNzG4 Do you count synaptonemal complex proteins, NPCs, etc that were initially identified using EM? https://t.c
Did you wonder how chromosomes adapt to oxygen availability? Under hypoxic conditions, #DNA adapts to a hollow, ring and rod-like configurations, which reverse back to the original structure when stress conditions cease. https://t.co/J6BLACNzG4 #Nobel20
@ReynaudEmmanuel @LennartHilbert @leonidmirny @Maurice_Y_Lee Another related observation is the decrease in active sites and the increase in condensation level. Under stress conditions, chromatin reversibly condenses into ring/rod-like structures with a s
@DocDellaire @HenriquesLab @D4V1D_4 Hoechst/DAPI need photoconversion (405 nm, 488 nm). Vibrant Violet and YOYO-1 work with one laser (491 nm). Key papers/protocols here: Hoechst and DAPI: https://t.co/t0JzYfjWgk VV and YOYO-1: https://t.co/3P3k1TxgQy h