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CRISPR/Cas9-mediated viral interference in plants

Overview of attention for article published in Genome Biology, November 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (85th percentile)

Mentioned by

news
4 news outlets
blogs
2 blogs
policy
1 policy source
twitter
28 X users
patent
1 patent
facebook
3 Facebook pages
wikipedia
1 Wikipedia page
googleplus
1 Google+ user

Citations

dimensions_citation
425 Dimensions

Readers on

mendeley
506 Mendeley
citeulike
2 CiteULike
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Title
CRISPR/Cas9-mediated viral interference in plants
Published in
Genome Biology, November 2015
DOI 10.1186/s13059-015-0799-6
Pubmed ID
Authors

Zahir Ali, Aala Abulfaraj, Ali Idris, Shakila Ali, Manal Tashkandi, Magdy M. Mahfouz

Abstract

The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.

X Demographics

X Demographics

The data shown below were collected from the profiles of 28 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 506 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 <1%
France 2 <1%
Brazil 2 <1%
Italy 1 <1%
Chile 1 <1%
Mexico 1 <1%
United Kingdom 1 <1%
Spain 1 <1%
Argentina 1 <1%
Other 0 0%
Unknown 494 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 93 18%
Student > Ph. D. Student 79 16%
Student > Bachelor 62 12%
Student > Master 59 12%
Student > Doctoral Student 31 6%
Other 62 12%
Unknown 120 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 221 44%
Biochemistry, Genetics and Molecular Biology 123 24%
Immunology and Microbiology 8 2%
Medicine and Dentistry 5 <1%
Computer Science 4 <1%
Other 18 4%
Unknown 127 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 74. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 April 2022.
All research outputs
#578,940
of 25,371,288 outputs
Outputs from Genome Biology
#343
of 4,467 outputs
Outputs of similar age
#8,569
of 293,328 outputs
Outputs of similar age from Genome Biology
#12
of 84 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 293,328 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 84 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.