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Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis

Overview of attention for article published in BMC Plant Biology, August 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Good Attention Score compared to outputs of the same age and source (79th percentile)

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7 X users

Citations

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114 Dimensions

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123 Mendeley
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Title
Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis
Published in
BMC Plant Biology, August 2018
DOI 10.1186/s12870-018-1387-1
Pubmed ID
Authors

Haroon Butt, Muhammad Jamil, Jian You Wang, Salim Al-Babili, Magdy Mahfouz

Abstract

Precision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering). We used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a reduced height, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography-mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient. Taken together, our results show the potential and feasibility of the use of the CRISPR/Cas9 system for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 123 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 123 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 24 20%
Student > Ph. D. Student 17 14%
Student > Master 16 13%
Student > Bachelor 13 11%
Student > Doctoral Student 8 7%
Other 21 17%
Unknown 24 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 55 45%
Biochemistry, Genetics and Molecular Biology 30 24%
Engineering 2 2%
Business, Management and Accounting 1 <1%
Nursing and Health Professions 1 <1%
Other 5 4%
Unknown 29 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2018.
All research outputs
#12,812,167
of 23,102,082 outputs
Outputs from BMC Plant Biology
#815
of 3,290 outputs
Outputs of similar age
#153,700
of 335,220 outputs
Outputs of similar age from BMC Plant Biology
#13
of 62 outputs
Altmetric has tracked 23,102,082 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,290 research outputs from this source. They receive a mean Attention Score of 3.0. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,220 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 62 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 79% of its contemporaries.